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Infection and Immunity, March 2000, p. 1696-1699, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Macropinocytosis as a Mechanism of Entry into Primary Human Urethral Epithelial Cells by Neisseria gonorrhoeae

Michael K. Zenni,1 Peter C. Giardina,2 Hillery A. Harvey,2 Jianqiang Shao,2 Margaret R. Ketterer,2 David M. Lubaroff,1 Richard D. Williams,1 and Michael A. Apicella2,*

Department of Urology1 and Department of Microbiology,2 University of Iowa, Iowa City, Iowa 52242

Received 12 July 1999/Returned for modification 1 September 1999/Accepted 17 November 1999

Gonococcal entry into primary human urethral epithelial cells (HUEC) can occur by macropinocytosis. Scanning and transmission electron microscopy revealed lamellipodia surrounding gonococci, and confocal laser scanning microscopy analysis showed organisms colocalized with Mr 70,000 fluorescein isothiocyanate-labeled dextran within the cells. Phosphoinositide 3-kinase inhibitors and an actin polymerization inhibitor prevented macropinocytic entry of gonococci into HUEC.

* Corresponding author. Mailing address: Department of Microbiology, University of Iowa School of Medicine, 51 Newton Road, Iowa City, IA 52242. Phone: (319) 335-7807. Fax: (319) 335-9006. E-mail: michael-apicella{at}uiowa.edu.

Infection and Immunity, March 2000, p. 1696-1699, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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