This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kumar, S.
Right arrow Articles by Kaslow, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kumar, S.
Right arrow Articles by Kaslow, D. C.

 Previous Article  |  Next Article 

Infection and Immunity, April 2000, p. 2215-2223, Vol. 68, No. 4
0019-9567/00/$04.00+0

Immunogenicity and Efficacy in Aotus Monkeys of Four Recombinant Plasmodium falciparum Vaccines in Multiple Adjuvant Formulations Based on the 19-Kilodalton C Terminus of Merozoite Surface Protein 1

Sanjai Kumar,1,* William Collins,2 Andrea Egan,1 Anjali Yadava,1 Olivier Garraud,1 Michael J. Blackman,3 Jose A. Guevara Patino,3 Carter Diggs,4 and David C. Kaslow1

Laboratory of Parasitic Diseases, National Institutes of Health, Bethesda, Maryland 208921; Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303332; Division of Parasitology, National Institute for Medical Research, London NW71AA, United Kingdom3; Malaria Vaccine Development Program, U.S. Agency for International Development, Washington, D.C. 205234

Received 20 October 1999/Returned for modification 6 December 1999/Accepted 12 January 2000

The immunogenicity and protective efficacy of four versions of recombinant C-terminal 19-kDa epidermal growth factor-like region of the major surface protein 1 (rMSP119) of Plasmodium falciparum was studied in Aotus monkeys. Vaccination with each of the four rMSP119 constructs elicited high levels of antibodies to MSP119 but only one construct, the 19-kDa fragment expressed as a secreted fusion protein from Saccharomyces cerevisiae (yP30P2MSP119), induced a high degree of protective immunity in Aotus nancymai against lethal P. falciparum challenge. Protective formulation required Freund's adjuvant; vaccination with yP30P2MSP119 in six other adjuvants that are suitable for human use induced lower levels of antibody response and no protection. These results emphasize the need to continue the search for an adjuvant that is comparable to Freund's adjuvant in potency and is safe for use in humans.

* Corresponding author. Present address: Malaria Program, Naval Medical Research Center, 503 Robert Grant Ave., Silver Spring, MD 20910-7500. E-mail: kumars{at}nmripo.nmri.nnmc.navy.mil.

Infection and Immunity, April 2000, p. 2215-2223, Vol. 68, No. 4
0019-9567/00/$04.00+0


This article has been cited by other articles:

  • Wipasa, J., Xu, H., Liu, X., Hirunpetcharat, C., Stowers, A., Good, M. F. (2009). Effect of Plasmodium yoelii Exposure on Vaccination with the 19-Kilodalton Carboxyl Terminus of Merozoite Surface Protein 1 and Vice Versa and Implications for the Application of a Human Malaria Vaccine. Infect. Immun. 77: 817-824 [Abstract] [Full Text]  
  • Carralot, J.-P., Lemmel, C., Stevanovic, S., Pascolo, S. (2008). Mass spectrometric identification of an HLA-A*0201 epitope from Plasmodium falciparum MSP-1. Int Immunol 20: 1451-1456 [Abstract] [Full Text]  
  • Ogun, S. A., Dumon-Seignovert, L., Marchand, J.-B., Holder, A. A., Hill, F. (2008). The Oligomerization Domain of C4-Binding Protein (C4bp) Acts as an Adjuvant, and the Fusion Protein Comprised of the 19-Kilodalton Merozoite Surface Protein 1 Fused with the Murine C4bp Domain Protects Mice against Malaria. Infect. Immun. 76: 3817-3823 [Abstract] [Full Text]  
  • Faber, B. W., Remarque, E. J., Morgan, W. D., Kocken, C. H. M., Holder, A. A., Thomas, A. W. (2007). Malaria Vaccine-Related Benefits of a Single Protein Comprising Plasmodium falciparum Apical Membrane Antigen 1 Domains I and II Fused to a Modified Form of the 19-Kilodalton C-Terminal Fragment of Merozoite Surface Protein 1. Infect. Immun. 75: 5947-5955 [Abstract] [Full Text]  
  • Shi, Q., Lynch, M. M., Romero, M., Burns, J. M. Jr. (2007). Enhanced Protection against Malaria by a Chimeric Merozoite Surface Protein Vaccine. Infect. Immun. 75: 1349-1358 [Abstract] [Full Text]  
  • Singh, S., Miura, K., Zhou, H., Muratova, O., Keegan, B., Miles, A., Martin, L. B., Saul, A. J., Miller, L. H., Long, C. A. (2006). Immunity to Recombinant Plasmodium falciparum Merozoite Surface Protein 1 (MSP1): Protection in Aotus nancymai Monkeys Strongly Correlates with Anti-MSP1 Antibody Titer and In Vitro Parasite-Inhibitory Activity.. Infect. Immun. 74: 4573-4580 [Abstract] [Full Text]  
  • Woehlbier, U., Epp, C., Kauth, C. W., Lutz, R., Long, C. A., Coulibaly, B., Kouyate, B., Arevalo-Herrera, M., Herrera, S., Bujard, H. (2006). Analysis of Antibodies Directed against Merozoite Surface Protein 1 of the Human Malaria Parasite Plasmodium falciparum. Infect. Immun. 74: 1313-1322 [Abstract] [Full Text]  
  • COLLINS, W. E., GALLAND, G. G., BARNWELL, J. W., UDHAYAKUMAR, V., SULLIVAN, J. S., NACE, D., TONGREN, J. E., WILLIAMS, T., ROBERTS, J., SHI, Y. P., LAL, A. A. (2005). PRELIMINARY OBSERVATIONS ON THE EFFICACY OF A RECOMBINANT MULTISTAGE PLASMODIUM FALCIPARUM VACCINE IN AOTUS NANCYMAI MONKEYS. Am J Trop Med Hyg 73: 686-693 [Abstract] [Full Text]  
  • Dutta, S., Kaushal, D. C., Ware, L. A., Puri, S. K., Kaushal, N. A., Narula, A., Upadhyaya, D. S., Lanar, D. E. (2005). Merozoite Surface Protein 1 of Plasmodium vivax Induces a Protective Response against Plasmodium cynomolgi Challenge in Rhesus Monkeys. Infect. Immun. 73: 5936-5944 [Abstract] [Full Text]  
  • Wykes, M. N., Zhou, Y.-H., Liu, X. Q., Good, M. F. (2005). Plasmodium yoelii Can Ablate Vaccine-Induced Long-Term Protection in Mice. J. Immunol. 175: 2510-2516 [Abstract] [Full Text]  
  • YOON, I.-K., ANGOV, E., LARSON, D., HEPPNER, D. G., CUMMINGS, J. F., STEWART, V. A. (2005). CHARACTERIZATION OF A HUMAN REFERENCE STANDARD FOR ANTIBODY TO PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN 142. Am J Trop Med Hyg 72: 714-718 [Abstract] [Full Text]  
  • Carvalho, L. J. M., Alves, F. A., Bianco, C. Jr., Oliveira, S. G., Zanini, G. M., Soe, S., Druilhe, P., Theisen, M., Muniz, J. A. P. C., Daniel-Ribeiro, C. T. (2005). Immunization of Saimiri sciureus Monkeys with a Recombinant Hybrid Protein Derived from the Plasmodium falciparum Antigen Glutamate-Rich Protein and Merozoite Surface Protein 3 Can Induce Partial Protection with Freund and Montanide ISA720 Adjuvants. CVI 12: 242-248 [Abstract] [Full Text]  
  • Sachdeva, S., Ahmad, G., Malhotra, P., Mukherjee, P., Chauhan, V. S. (2004). Comparison of Immunogenicities of Recombinant Plasmodium vivax Merozoite Surface Protein 1 19- and 42-Kilodalton Fragments Expressed in Escherichia coli. Infect. Immun. 72: 5775-5782 [Abstract] [Full Text]  
  • Corran, P. H., O'Donnell, R. A., Todd, J., Uthaipibull, C., Holder, A. A., Crabb, B. S., Riley, E. M. (2004). The Fine Specificity, but Not the Invasion Inhibitory Activity, of 19-Kilodalton Merozoite Surface Protein 1-Specific Antibodies Is Associated with Resistance to Malarial Parasitemia in a Cross-Sectional Survey in The Gambia. Infect. Immun. 72: 6185-6189 [Abstract] [Full Text]  
  • Pan, W., Huang, D., Zhang, Q., Qu, L., Zhang, D., Zhang, X., Xue, X., Qian, F. (2004). Fusion of Two Malaria Vaccine Candidate Antigens Enhances Product Yield, Immunogenicity, and Antibody-Mediated Inhibition of Parasite Growth In Vitro. J. Immunol. 172: 6167-6174 [Abstract] [Full Text]  
  • Kumar, S., Jones, T. R., Oakley, M. S., Zheng, H., Kuppusamy, S. P., Taye, A., Krieg, A. M., Stowers, A. W., Kaslow, D. C., Hoffman, S. L. (2004). CpG Oligodeoxynucleotide and Montanide ISA 51 Adjuvant Combination Enhanced the Protective Efficacy of a Subunit Malaria Vaccine. Infect. Immun. 72: 949-957 [Abstract] [Full Text]  
  • Singh, S., Kennedy, M. C., Long, C. A., Saul, A. J., Miller, L. H., Stowers, A. W. (2003). Biochemical and Immunological Characterization of Bacterially Expressed and Refolded Plasmodium falciparum 42-Kilodalton C-Terminal Merozoite Surface Protein 1. Infect. Immun. 71: 6766-6774 [Abstract] [Full Text]  
  • SULLIVAN, J. S., SULLIVAN, J. J., WILLIAMS, A., GRADY, K. K., BOUNNGASENG, A., HUBER, C. S., NACE, D., WILLIAMS, T., GALLAND, G. G., BARNWELL, J. W., COLLINS, W. E. (2003). ADAPTATION OF A STRAIN OF PLASMODIUM FALCIPARUM FROM GHANA TO AOTUS LEMURINUS GRISEIMEMBRA, A. NANCYMAI, AND A. VOCIFERANS MONKEYS. Am J Trop Med Hyg 69: 593-600 [Abstract] [Full Text]  
  • Polley, S. D., Tetteh, K. K. A., Cavanagh, D. R., Pearce, R. J., Lloyd, J. M., Bojang, K. A., Okenu, D. M. N., Greenwood, B. M., McBride, J. S., Conway, D. J. (2003). Repeat Sequences in Block 2 of Plasmodium falciparum Merozoite Surface Protein 1 Are Targets of Antibodies Associated with Protection from Malaria. Infect. Immun. 71: 1833-1842 [Abstract] [Full Text]  
  • Stowers, A. W., Kennedy, M. C., Keegan, B. P., Saul, A., Long, C. A., Miller, L. H. (2002). Vaccination of Monkeys with Recombinant Plasmodium falciparum Apical Membrane Antigen 1 Confers Protection against Blood-Stage Malaria. Infect. Immun. 70: 6961-6967 [Abstract] [Full Text]  
  • Pang, A. L. Y., Hashimoto, C. N., Tam, L. Q., Meng, Z. Q., Hui, G. S. N., Ho, W. K. K. (2002). In Vivo Expression and Immunological Studies of the 42-Kilodalton Carboxyl-Terminal Processing Fragment of Plasmodium falciparum Merozoite Surface Protein 1 in the Baculovirus-Silkworm System. Infect. Immun. 70: 2772-2779 [Abstract] [Full Text]  
  • Qian, F., Pan, W. (2002). Construction of a tetR-Integrated Salmonella enterica Serovar Typhi CVD908 Strain That Tightly Controls Expression of the Major Merozoite Surface Protein of Plasmodium falciparum for Applications in Human Vaccine Production. Infect. Immun. 70: 2029-2038 [Abstract] [Full Text]  
  • Near, K. A., Stowers, A. W., Jankovic, D., Kaslow, D. C. (2002). Improved Immunogenicity and Efficacy of the Recombinant 19-Kilodalton Merozoite Surface Protein 1 by the Addition of Oligodeoxynucleotide and Aluminum Hydroxide Gel in a Murine Malaria Vaccine Model. Infect. Immun. 70: 692-701 [Abstract] [Full Text]  
  • O'Donnell, R. A., de Koning-Ward, T. F., Burt, R. A., Bockarie, M., Reeder, J. C., Cowman, A. F., Crabb, B. S. (2001). Antibodies against Merozoite Surface Protein (Msp)-119 Are a Major Component of the Invasion-Inhibitory Response in Individuals Immune to Malaria. JEM 193: 1403-1412 [Abstract] [Full Text]  
  • Moreno, R., Poltl-Frank, F., Stuber, D., Matile, H., Mutz, M., Weiss, N. A., Pluschke, G. (2001). Rhoptry-Associated Protein 1-Binding Monoclonal Antibody Raised against a Heterologous Peptide Sequence Inhibits Plasmodium falciparum Growth In Vitro. Infect. Immun. 69: 2558-2568 [Abstract] [Full Text]  
  • Stowers, A. W., Cioce, V., Shimp, R. L., Lawson, M., Hui, G., Muratova, O., Kaslow, D. C., Robinson, R., Long, C. A., Miller, L. H. (2001). Efficacy of Two Alternate Vaccines Based on Plasmodium falciparum Merozoite Surface Protein 1 in an Aotus Challenge Trial. Infect. Immun. 69: 1536-1546 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»