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Infection and Immunity, April 2000, p. 2328-2332, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Stable Expression of a New Chimeric Fluorescent Reporter in the Human Malaria Parasite Plasmodium falciparum

Madhusudan Kadekoppala, Kimberly Kline, Thomas Akompong, and Kasturi Haldar*

Departments of Pathology and Microbiology-Immunology, Northwestern University School of Medicine, Chicago, Illinois 60611

Received 15 October 1999/Accepted 1 January 2000

Stable transfection of a new, chimeric reporter in the human malaria parasite Plasmodium falciparum confers green fluorescence and methotrexate resistance that can be quantitated by Western blotting and flow cytometry. This provides a sensitive, live reporter for exploitation of genomic and high-throughput assays for the identification of new pathogenic determinants.

* Corresponding author. Mailing address: Departments of Pathology and Microbiology-Immunology, Northwestern University School of Medicine, 303 E. Chicago Ave., Chicago, IL 60611. Phone: (312) 503-0224. Fax: (312) 503-8240. E-mail: k-haldar{at}nwu.edu.

Infection and Immunity, April 2000, p. 2328-2332, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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