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Infection and Immunity, May 2000, p. 2560-2565, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Immunization with Recombinant Helicobacter pylori Urease in Specific-Pathogen-Free Rhesus Monkeys (Macaca mulatta)

Jay V. Solnick,1,* Don R. Canfield,2 Lori M. Hansen,1 and Sima Z. Torabian1

Departments of Internal Medicine (Division of Infectious Diseases) and Medical Microbiology and Immunology, University of California, Davis School of Medicine,1 and California Regional Primate Research Center,2 Davis, California 95616

Received 9 September 1999/Returned for modification 30 November 1999/Accepted 1 February 2000

Immunization with urease can protect mice from challenge with Helicobacter pylori, though results vary depending on the particular vaccine, challenge strain, and method of evaluation. Unlike mice, rhesus monkeys are naturally colonized with H. pylori and so may provide a better estimate of vaccine efficacy in humans. The purpose of this study was to examine the effectiveness of H. pylori urease as a vaccine in specific-pathogen (H. pylori)-free rhesus monkeys. Monkeys raised from birth and documented to be free of H. pylori were vaccinated with orogastric (n = 4) or intramuscular (n = 5) urease. Two control monkeys were sham vaccinated. All monkeys were challenged with a rhesus monkey-derived strain of H. pylori, and the effects of vaccination were evaluated by use of quantitative cultures of gastric tissue, histology, and measurement of serum immunoglobulin G (IgG) and salivary IgA. Despite a humoral immune response, all monkeys were infected after H. pylori challenge, and there were no differences in the density of colonization. Immunization with urease therefore does not fully protect against challenge with H. pylori. An effective vaccine to prevent H. pylori infection will require different or more likely additional antigens, as well as improvements in the stimulation of the host immune response.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, University of California at Davis, Davis, CA 95616. Phone: (530) 752-1333. Fax: (530) 752-8692. E-mail: jvsolnick{at}ucdavis.edu.

Infection and Immunity, May 2000, p. 2560-2565, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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