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Infection and Immunity, May 2000, p. 2566-2572, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Outer Membrane Protein A, Peptidoglycan-Associated Lipoprotein,
and Murein Lipoprotein Are Released by Escherichia coli
Bacteria into Serum
Judith
Hellman,1,*
Paul M.
Loiselle,2
Megan M.
Tehan,1
Jennifer E.
Allaire,2
Lenora A.
Boyle,3
James T.
Kurnick,3
David M.
Andrews,3
Kwang
Sik
Kim,4 and
H. Shaw
Warren1,5
Departments of Anesthesia and
Critical Care,2 Infectious
Diseases,1
Pathology,3 and
Pediatrics,5 Massachusetts General Hospital and
Harvard Medical School, Boston, Massachusetts, and Department
of Pediatrics, Childrens Hospital of Los Angeles and University of
Southern California School of Medicine, Los Angeles,
California4
Complexes containing lipopolysaccharide (LPS) and three outer
membrane proteins (OMPs) are released by gram-negative bacteria incubated in human serum and into the circulation in an experimental model of sepsis. The same OMPs are bound by immunoglobulin G (IgG) in
the cross-protective antiserum raised to Escherichia coli
J5 (anti-J5 IgG). This study was performed to identify the three OMPs.
The 35-kDa OMP was identified as outer membrane protein A (OmpA) by
immunoblotting studies using OmpA-deficient bacteria and recombinant
OmpA protein. The 18-kDa OMP was identified as peptidoglycan-associated
lipoprotein (PAL) based on peptide sequences from the purified protein
and immunoblotting studies using PAL-deficient bacteria. The 5- to
9-kDa OMP was identified as murein lipoprotein (MLP) based on
immunoblotting studies using MLP-deficient bacteria. The studies
identify the OMPs released into human serum and into the circulation in
an experimental model of sepsis as OmpA, PAL, and MLP.
*
Corresponding author. Mailing address: Infectious
Disease Unit, 5th Floor, 149 Thirteenth St., Charlestown, MA
02129. Phone: (617) 724-3104 or 724-4959. Fax: (617) 726-4176. E-mail: hellman{at}etherdome.mgh.harvard.edu.
Infection and Immunity, May 2000, p. 2566-2572, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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