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Infection and Immunity, May 2000, p. 2797-2803, Vol. 68, No. 5
Serviço de Imunologia, Hospital
Universitário Prof. Edgard Santos, Universidade Federal da Bahia,
Bahia, Brazil1; Department of
Microbiology, Immunology and Parasitology, Cornell University College
of Veterinary Medicine, Ithaca, New York2;
Department of Immunology and Infectious Diseases, Harvard
School of Public Health, Boston,
Massachusetts3; and Department of
Pharmacology and Molecular Sciences, The Johns Hopkins University
School of Medicine, Baltimore, Maryland4
Received 26 July 1999/Returned for modification 19 October
1999/Accepted 16 February 2000
To determine the naturally occurring immunological responses to the
Schistosoma mansoni antigens paramyosin, IrV-5,
Sm-23 (MAP-3), and triose phosphate isomerase (MAP-4), a total of 119 subjects from an area of endemicity for schistosomiasis, including "resistant" subjects (n = 17) were evaluated.
Specific immunoglobulin G1 (IgG1), IgG2, IgG3, IgG4, and
IgA levels for each of the antigens and the cytokine profile
in culture supernatants from antigen-stimulated peripheral
blood mononuclear cells (PBMC) were determined. Although all the
subjects had a high degree of contaminated water exposure, their
infection levels were variable (0 to 1,128 eggs/g of stool). There were
direct correlations between infection levels and levels of SWAP-
and paramyosin-specific IgG1 and IgG4 (P < 0.05). However, an inverse correlation between infection levels and
specific IgG2 to IrV-5 (P < 0.01) was observed. The
evaluation of the cytokine profile (interleukin 5 [IL-5],
IL-10, gamma interferon [IFN-
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Human Immune Responses to Schistosoma
mansoni Vaccine Candidate Antigens
and
], and tumor necrosis factor
alpha) in response to these antigens showed inverse correlations
between the degree of infection and IFN- levels in PBMC supernatants
stimulated with paramyosin (P < 0.05) and IrV-5
(P < 0.01). Additionally, inverse correlations between the degree of infection and IL-5 levels in MAP-3- and MAP-4-stimulated PBMC supernatants (P < 0.01) were
found. Logistic regression analysis was performed to adjust
the results of cytokine profile by age. IL-5 production in
MAP-3-stimulated PBMC supernatants was associated with lower
infection levels (odds ratio = 11.2 [95% confidence interval,
2.7 to 45.8]).
*
Corresponding author. Mailing address: Serviço de
Imunologia, Hospital Universitário Prof. Edgard Santos,
Universidade Federal da Bahia, Bahia CEP 40110-160, Brazil. Phone:
(55-71) 2377353. Fax: (55-71) 2457110. E-mail: edgar{at}ufba.br.
Deceased.
Infection and Immunity, May 2000, p. 2797-2803, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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