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Infection and Immunity, May 2000, p. 3056-3060, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

R1 Region of P97 Mediates Adherence of Mycoplasma hyopneumoniae to Swine Cilia

F. Chris Minion,* Cary Adams, and Tsungda Hsudagger

Department of Veterinary Microbiology and Preventive Medicine, Veterinary Medical Research Institute, Iowa State University, Ames, Iowa 50011

Received 23 November 1999/Returned for modification 11 January 2000/Accepted 27 January 2000

Adherence of Mycoplasma hyopneumoniae to the swine respiratory tract is mediated by the membrane protein P97. This protein is located on the outer membrane surface, and its role in adherence has been firmly established. The general region of P97 that mediates adherence to swine cilia is thought to be the R1 region near the carboxy terminus of the protein, but it was not clear if this region could mediate adherence to swine cilia independently of other P97 sequences. To examine this in more detail, a series of R1 repeat sequences containing different numbers of repeating units cloned in frame with lacZ was used to produce R1-beta -galactosidase fusion proteins. These proteins were then tested for adherence to swine cilia and for reactivity to the adherence-blocking monoclonal antibody F2G5 and convalescent-phase swine sera. In this way it was possible to accurately define the cilium binding epitope of P97 and the minimal epitope recognized by antibody. Our results indicate that eight R1 repeating units are required for cilium binding and that three repeating units are needed for antibody recognition. These results could lead to more effective therapeutic measures against this important swine pathogen.

* Corresponding author. Mailing address: Iowa State University, Veterinary Medical Research Institute, 1802 Elwood Dr., Ames, IA 50011. Phone: (515) 294-6347. Fax: (515) 294-1401. E-mail: fcminion{at}iastate.edu.

dagger Present address: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461.

Infection and Immunity, May 2000, p. 3056-3060, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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