This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Silva, C. L.
Right arrow Articles by Lowrie, D. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Silva, C. L.
Right arrow Articles by Lowrie, D. B.

 Previous Article  |  Next Article 

Infection and Immunity, June 2000, p. 3269-3274, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification and Characterization of Murine Cytotoxic T Cells That Kill Mycobacterium tuberculosis

Celio L. Silva1,* and Douglas B. Lowrie2

Department of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Brazil,1 and National Institute for Medical Research, London, United Kingdom2

Received 27 October 1999/Returned for modification 13 December 1999/Accepted 14 March 2000

As we seek to develop and evaluate new vaccines against tuberculosis, it is desirable that we understand the mechanisms of protective immunity in our models. Adoptive transfer of protection with hsp65-specific T-cell clones from infected or vaccinated mice into naïve mice had indicated that cytotoxic T cells can make a major contribution to protection. We characterized 28 CD4+ CD8- and 28 CD4- CD8+ hsp65-specific T-cell clones derived from infected or vaccinated mice. Half of the CD4+ CD8- and 64% of the CD4- CD8+ clones were cytotoxic. Cytotoxicity was associated with high expression of CD44 and gamma interferon production. Most (86%) of the cytotoxic CD4+ CD8- clones lysed target cells via the Fas-FasL pathway, and most (83%) of the cytotoxic CD4- CD8+ clones lysed target cells via cytotoxic granules. Only the clones using the granule-mediated pathway caused substantial loss of viability of virulent Mycobacterium tuberculosis during lysis of infected macrophages, and the degree of killing closely correlated with the availability of granule marker enzyme activity. Granule-mediated cytotoxicity thus may have a key role in protection against tuberculosis by delivering mycobactericidal granule contents.

* Corresponding author. Mailing address: Department of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, 14049-900, Ribeirão Preto, SP, Brazil. Phone: 55 16 633 3035. Fax: 55 16 633 6631. E-mail: clsilva{at}beverly.fmrp.usp.br.

Infection and Immunity, June 2000, p. 3269-3274, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Woodworth, J. S., Wu, Y., Behar, S. M. (2008). Mycobacterium tuberculosis-Specific CD8+ T Cells Require Perforin to Kill Target Cells and Provide Protection In Vivo. J. Immunol. 181: 8595-8603 [Abstract] [Full Text]  
  • Andersson, J., Samarina, A., Fink, J., Rahman, S., Grundstrom, S. (2007). Impaired Expression of Perforin and Granulysin in CD8+ T Cells at the Site of Infection in Human Chronic Pulmonary Tuberculosis. Infect. Immun. 75: 5210-5222 [Abstract] [Full Text]  
  • Oliaro, J., Dudal, S., Liautard, J., Andrault, J.-B., Liautard, J.-P., Lafont, V. (2005). V{gamma}9V{delta}2 T cells use a combination of mechanisms to limit the spread of the pathogenic bacteria Brucella. J. Leukoc. Biol. 77: 652-660 [Abstract] [Full Text]  
  • Kamath, A. B., Woodworth, J., Xiong, X., Taylor, C., Weng, Y., Behar, S. M. (2004). Cytolytic CD8+ T Cells Recognizing CFP10 Are Recruited to the Lung after Mycobacterium tuberculosis Infection. JEM 200: 1479-1489 [Abstract] [Full Text]  
  • Gehring, A. J., Dobos, K. M., Belisle, J. T., Harding, C. V., Boom, W. H. (2004). Mycobacterium tuberculosis LprG (Rv1411c): A Novel TLR-2 Ligand That Inhibits Human Macrophage Class II MHC Antigen Processing. J. Immunol. 173: 2660-2668 [Abstract] [Full Text]  
  • Tobian, A. A. R., Potter, N. S., Ramachandra, L., Pai, R. K., Convery, M., Boom, W. H., Harding, C. V. (2003). Alternate Class I MHC Antigen Processing Is Inhibited by Toll-Like Receptor Signaling Pathogen-Associated Molecular Patterns: Mycobacterium tuberculosis 19-kDa Lipoprotein, CpG DNA, and Lipopolysaccharide. J. Immunol. 171: 1413-1422 [Abstract] [Full Text]  
  • Lazarevic, V., Flynn, J. (2002). CD8+ T Cells in Tuberculosis. Am. J. Respir. Crit. Care Med. 166: 1116-1121 [Full Text]  
  • Raman, S., Song, T., Puyang, X., Bardarov, S., Jacobs, W. R. Jr., Husson, R. N. (2001). The Alternative Sigma Factor SigH Regulates Major Components of Oxidative and Heat Stress Responses in Mycobacterium tuberculosis. J. Bacteriol. 183: 6119-6125 [Abstract] [Full Text]  
  • Canaday, D. H., Wilkinson, R. J., Li, Q., Harding, C. V., Silver, R. F., Boom, W. H. (2001). CD4+ and CD8+ T Cells Kill Intracellular Mycobacterium tuberculosis by a Perforin and Fas/Fas Ligand-Independent Mechanism. J. Immunol. 167: 2734-2742 [Abstract] [Full Text]  
  • Serbina, N. V., Flynn, J. L. (2001). CD8+ T Cells Participate in the Memory Immune Response to Mycobacterium tuberculosis. Infect. Immun. 69: 4320-4328 [Abstract] [Full Text]  
  • Xing, Z., Zganiacz, A., Wang, J., Sharma, S. K. (2001). Enhanced Protection Against Fatal Mycobacterial Infection in SCID Beige Mice by Reshaping Innate Immunity with IFN-{{gamma}} Transgene. J. Immunol. 167: 375-383 [Abstract] [Full Text]  
  • Coler, R. N., Campos-Neto, A., Ovendale, P., Day, F. H., Fling, S. P., Zhu, L., Serbina, N., Flynn, J. L., Reed, S. G., Alderson, M. R. (2001). Vaccination with the T Cell Antigen Mtb 8.4 Protects Against Challenge with Mycobacterium tuberculosis. J. Immunol. 166: 6227-6235 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»