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Infection and Immunity, June 2000, p. 3349-3351, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Model of Infected Burn Wounds Using Escherichia coli O18:K1:H7 for the Study of Gram-Negative Bacteremia and Sepsis

Nathan A. Busch,1,dagger Emily M. Zanzot,2,3,Dagger Paul M. Loiselle,2,3 Edward A. Carter,1,3 Jennifer E. Allaire,2 Martin L. Yarmush,1,3 and H. Shaw Warren2,3,*

Center for Engineering in Medicine and Surgical Services,1 and Infectious Disease Unit,2 Massachusetts General Hospital, Harvard Medical School, and Infectious Disease Unit, The Shriners Burns Research Institute,3 Boston, Massachusetts 02114

Received 10 September 1999/Returned for modification 10 January 2000/Accepted 24 March 2000

A difficulty that has emerged in the development and preclinical evaluation of adjuvant therapies for gram-negative sepsis is the lack of easily studied animal models that closely mimic human infection. An objective of this study was to adapt a previously described model of infection in burned mice to rats with a defined bacterial strain of Escherichia coli. Challenge with two colonies of live E. coli O18:K1:H7 bacteria into an 8% full-thickness burn of the dorsal skin surface of rats produced predictable bacteremia at 24 to 48 h and 80 to 100% mortality at 3 to 4 days. E. coli O18:K1:H7 was approximately 10-million-fold more virulent than several other gram-negative bacterial strains. The model should be a useful tool in studying the pathogenicity of burn wound infections and in evaluating the efficacy of novel adjuvant therapies for gram-negative sepsis.

* Corresponding author. Mailing address: Infectious Disease Unit, 5th floor, Massachusetts General Hospital, 149 13th St., Charlestown, MA 02129. Phone: (617) 726-5774. Fax: (617) 726-5411. E-mail: warren{at}helix.mgh.harvard.edu.

dagger Present address: Department of Biochemistry, University of Minnesota, St. Paul, Minn.

Dagger Present address: Division of Speech and Hearing Sciences, University of North Carolina at Chapel Hill, Chapel Hill, N.C.

Infection and Immunity, June 2000, p. 3349-3351, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hellman, J., Shaw Warren, H. (2001). Outer membrane protein A (OmpA), peptidoglycan-associated lipoprotein (PAL), and murein lipoprotein (MLP) are released in experimental Gram-negative sepsis. Innate Immunity 7: 69-72 [Abstract]  


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