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Infection and Immunity, June 2000, p. 3754-3757, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cell Specificity of Helicobacter pylori Cytotoxin Is Determined by a Short Region in the Polymorphic Midregion

Xuhuai Ji,1,2 Thomas Fernandez,1 Daniela Burroni,1 Cristina Pagliaccia,1 John C. Atherton,3 Jean-Marc Reyrat,1,dagger Rino Rappuoli,1 and John L. Telford1,*

Department of Molecular Biology, IRIS, Chiron Vaccines, Siena 53100, Italy1; Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China2; and Division of Gastroenterology, University Hospital, University of Nottingham, Nottingham NG7 2UH, United Kingdom3

Received 22 December 1999/Returned for modification 28 January 2000/Accepted 7 March 2000

There are two alleles of the vacuolating cytotoxin gene from Helicobacter pylori, which code for toxins with different cell specificities. By analyzing the phenotypes of natural and artificial chimeras between the two forms of the protein, we have delimited a short stretch of amino acids which determine the cell specificity.

* Corresponding author. Mailing address: IRIS, Chiron Vaccines, Via Fiorentina 1, Siena 53100, Italy. Phone: 39-0577-243470. Fax: 39-0577-243564. E-mail: John_Telford{at}biocine.it.

dagger Present address: Unité de Génétique Mycobactérienne, Institut Pasteur, 75724 Paris Cedex 15, France.

Infection and Immunity, June 2000, p. 3754-3757, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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