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Infection and Immunity, June 2000, p. 3754-3757, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Cell Specificity of Helicobacter pylori
Cytotoxin Is Determined by a Short Region in the Polymorphic
Midregion
Xuhuai
Ji,1,2
Thomas
Fernandez,1
Daniela
Burroni,1
Cristina
Pagliaccia,1
John C.
Atherton,3
Jean-Marc
Reyrat,1,
Rino
Rappuoli,1 and
John L.
Telford1,*
Department of Molecular Biology, IRIS, Chiron
Vaccines, Siena 53100, Italy1;
Department of Gastroenterology, Changhai Hospital, Second
Military Medical University, Shanghai 200433, China2; and Division of
Gastroenterology, University Hospital, University of Nottingham,
Nottingham NG7 2UH, United Kingdom3
Received 22 December 1999/Returned for modification 28 January
2000/Accepted 7 March 2000
There are two alleles of the vacuolating cytotoxin gene from
Helicobacter pylori, which code for toxins with different
cell specificities. By analyzing the phenotypes of natural and
artificial chimeras between the two forms of the protein, we have
delimited a short stretch of amino acids which determine the cell specificity.
*
Corresponding author. Mailing address: IRIS, Chiron
Vaccines, Via Fiorentina 1, Siena 53100, Italy. Phone: 39-0577-243470. Fax: 39-0577-243564. E-mail: John_Telford{at}biocine.it.

Present address: Unité de Génétique
Mycobactérienne, Institut Pasteur, 75724 Paris Cedex 15,
France.
Infection and Immunity, June 2000, p. 3754-3757, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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