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Infection and Immunity, June 2000, p. 3772-3775, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Functional cra Gene Is Required for Salmonella enterica Serovar Typhimurium Virulence in BALB/c Mice

James H. Allen,1 Maryjane Utley,1 Han van den Bosch,2 Piet Nuijten,2 Maarten Witvliet,2 Beth A. McCormick,3 Karen A. Krogfelt,4 Tine Rask Licht,5 Derek Brown,6 Michael Mauel,1 Mary P. Leatham,1 David C. Laux,1 and Paul S. Cohen1,*

Department of Biochemistry, Microbiology, and Molecular Genetics, University of Rhode Island, Kingston, Rhode Island 028811; Bacteriological and Immunological Research and Development, Intervet International, 5830 AA Boxmeer, The Netherlands2; Combined Program in Pediatric Gastroenterology and Nutrition, Harvard Medical School, Massachusetts General Hospital, Charlestown, Massachusetts 021203; Department of Gastrointestinal Infections, Statens Seruminstitut, 2300 Copenhagen,4 and Department of Microbiology, Technical University of Denmark, DK-2800 Lyngby,5 Denmark; and Scottish Salmonella Reference Laboratory, Stobhill NHS Trust, Glasgow G21 3UW, Scotland6

Received 16 December 1999/Returned for modification 17 February 2000/Accepted 6 March 2000

A minitransposon mutant of Salmonella enterica serovar Typhimurium SR-11, SR-11 Fad-, is unable to utilize gluconeogenic substrates as carbon sources and is avirulent and immunogenic when administered perorally to BALB/c mice (M. J. Utley et al., FEMS Microbiol. Lett., 163:129-134, 1998). Here, evidence is presented that the mutation in SR-11 Fad- that renders the strain avirulent is in the cra gene, which encodes the Cra protein, a regulator of central carbon metabolism.

* Corresponding author. Mailing address: Department of Biochemistry, Microbiology, and Molecular Genetics, Morrill Science Bldg., 45 Lower College Rd., University of Rhode Island, Kingston, RI 02881. Phone: (401) 874-5920. Fax: (401) 874-2202. E-mail: pco1697u{at}postoffice.uri.edu.

Infection and Immunity, June 2000, p. 3772-3775, Vol. 68, No. 6
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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