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Infection and Immunity, July 2000, p. 3840-3847, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Measurement of T-Cell-Derived Antigen Binding Molecules and Immunoglobulin G Specific to Candida albicans Mannan in Sera of Patients with Recurrent Vulvovaginal Candidiasis

Colin H. Little,1,* George M. Georgiou,2 Alex Marceglia,3 Henry Ogedgebe,4 Robert E. Cone,4 and Danielle Mazza5

Specialist Allergist, Mt. Waverley,1 Department of Immunology, Royal Children's Hospital, Parkville,2 Melbourne Sexual Health Centre, Carlton,3 and Family Planning Victoria Inc., Box Hill,5 Melbourne, Australia, and Department of Pathology, University of Connecticut Health Center, Farmington, Connecticut4

Received 13 September 1999/Returned for modification 17 November 1999/Accepted 5 April 2000

Immunoglobulin G (IgG) and T-cell-derived antigen binding molecules (TABM) specific to whole Candida extract and to Candida-derived mannans prepared by both the cetryltrimethylammonium bromide (CTAB) and alkaline degradation (PEAT) methods were measured in the sera of women with vulvovaginal candidiasis and controls. In the patients there were significantly higher levels of IgG to both CTAB and PEAT mannans and of TABM to CTAB mannan. TABM specific to CTAB mannan was purified from the serum of a patient with a high titer of this TABM. The purified TABM bound specifically to CTAB mannan and to other yeast and mold extracts. This TABM preparation was associated with transforming growth factor beta 2 (TGF-beta 2), and on specific binding to mannan there was a marked increase in the level of detectable TGF-beta 2. This increase in TGF-beta 2 level was critically dependent on the relative concentrations of the purified TABM and mannan, being smallest when either was in excess. The TABM specific to CTAB mannan was also shown to inhibit Candida-stimulated gamma interferon production. The results suggest that CTAB mannan-specific TABM may increase susceptibility to vulvovaginal candidiasis in association with a shift in the immune response to the Th2 type.

* Corresponding author. Mailing address: 324 Stephensons Rd., Mt. Waverley, Melbourne, Victoria 3149, Australia. Phone: 61 3 9888 1345. Fax: 61 3 9888 1369. E-mail: littlec{at}bluep.com.

Infection and Immunity, July 2000, p. 3840-3847, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kosonen, J., Rantala, A., Little, C. H., Lintu, P., Harjamaki, P.-R., Georgiou, G. M., Cone, R. E., Savolainen, J. (2006). Increased Levels of Candida albicans Mannan-Specific T-Cell-Derived Antigen Binding Molecules in Patients with Invasive Candidiasis. CVI 13: 467-474 [Abstract] [Full Text]  
  • Cone, R. E., Georgiou, G. M., Little, C. H. (2002). Soluble T-Lymphocyte Antigen-Specific Immunoproteins: A Progress Report. Exp. Biol. Med. 227: 438-444 [Abstract] [Full Text]  


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