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Infection and Immunity, July 2000, p. 4323-4326, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Motility Is Required To Initiate Host Cell Invasion by Yersinia enterocolitica

Glenn M. Young,1,dagger Julie L. Badger,2,Dagger and Virginia L. Miller1,2,3,*

Department of Molecular Microbiology,1 and Division of Infectious Disease, Department of Pediatrics,3 Washington University School of Medicine and St. Louis Children's Hospital, St. Louis, Missouri 63110, and Department of Microbiology and Molecular Genetics, University of California at Los Angeles, Los Angeles, California 900952

Received 3 January 2000/Returned for modification 6 March 2000/Accepted 29 March 2000

Invasin-mediated invasion of host cells by the pathogen Yersinia enterocolitica was shown to be affected by flagellar-dependent motility. Motility appears to be required to ensure the bacterium migrates to and contacts the host cell. Nonmotile strains of Y. enterocolitica were less invasive than motile strains, but the reduction in invasion could be overcome by artificially bringing the bacteria into host cell contact by centrifugation. Mutations in known regulatory genes of the flagellar regulon, flhDC and fliA, resulted in less inv expression but did not have a significant effect on invasin levels. However, invasin levels were reduced for strains that harbored flhDC on a multicopy plasmid, apparently as a result of increased proteolysis of invasin.

* Corresponding author. Mailing address: Department of Molecular Microbiology, Washington University School of Medicine, Campus Box 8230, 660 S. Euclid Ave., St. Louis, MO 63110-1093. Phone: (314) 747-2132. Fax: (314) 747-2135. E-mail: virginia{at}borcim.wustl.edu.

dagger Present address: Graduate Faculty of Microbiology, and Food Science & Technology, Department of Food Science and Technology, University of California, Davis, CA 95616.

Dagger Present address: Division of Infectious Diseases, Childrens Hospital Los Angeles, Los Angeles, CA 90027.

Infection and Immunity, July 2000, p. 4323-4326, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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