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Infection and Immunity, July 2000, p. 4335-4339, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Adherence of Isogenic Flagellum-Negative Mutants of Helicobacter pylori and Helicobacter mustelae to Human and Ferret Gastric Epithelial Cells

Marguerite Clyne,1,2,* Tadhg Ocroinin,1,2 Sebastian Suerbaum,3,dagger Christine Josenhans,3,Dagger and Brendan Drumm1,2

Department of Paediatrics and The Conway Institute of Molecular and Biomedical Research, University College Dublin, Belfield, Dublin 4,1 and The Childrens Research Centre, Our Ladys Hospital for Sick Children, Crumlin, Dublin 12,2 Ireland, and Ruhr-Universitat Bochum, D-4630 Bochum, Germany3

Received 19 January 2000/Returned for modification 21 February 2000/Accepted 11 April 2000

Isogenic flagellum-negative mutants of Helicobacter pylori and Helicobacter mustelae were screened for their ability to adhere to primary human and ferret gastric epithelial cells, respectively. We also evaluated the adherence of an H. pylori strain with a mutation in the flbA gene, a homologue of the flbF/lcrD family of genes known to be involved in the regulation of H. pylori flagellar biosynthesis. H. pylori and H. mustelae mutants deficient in production of FlaA or FlaB and mutants deficient in the production of both FlaA and FlaB showed no reduction in adherence to primary human or ferret gastric epithelial cells compared with the wild-type parental strains. However, adherence of the H. pylori flbA mutant to human gastric cells was significantly reduced compared to the adherence of the wild-type strain. These results show that flagella do not play a direct role in promoting adherence of H. pylori or H. mustelae to gastric epithelial cells. However, genes involved in the regulation of H. pylori flagellar biosynthesis may also regulate the production of an adhesin.

* Corresponding author. Mailing address: The Childrens Research Centre, Our Ladys Hospital for Sick Children, Crumlin, Dublin 12, Ireland. Phone: 353-1-4096594. Fax: 353-1-4555307. E-mail: MARGUERITE.CLYNE{at}ucd.ie.

dagger Present address: Institute of Hygiene and Microbiology, University of Wuerzburg, D-97080 Wuerzburg, Germany.

Dagger Present address: Microbial Pathogenesis Unit, Institute of Cellular Pathology, Universite Catholique de Louvain, B-1200 Bruxelles, Belgium.

Infection and Immunity, July 2000, p. 4335-4339, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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