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Infection and Immunity, July 2000, p. 4349-4353, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Adjuvant Activity of a Nontoxic Mutant of Escherichia coli Heat-Labile Enterotoxin on Systemic and Mucosal Immune Responses Elicited against a Heterologous Antigen Carried by a Live Salmonella enterica Serovar Typhimurium Vaccine Strain

Heloisa C. R. Guillobel,1,2,3 Joana I. Carinhanha,1 Lucia Cárdenas,4 John D. Clements,4 Darcy F. de Almeida,1 and Luís C. S. Ferreira1,*

Laboratório de Fisiologia Celular, Instituto de Biofisica Carlos Chagas Filho, CCS, Universidade Federal do Rio de Janeiro, 21941-590 Rio de Janeiro,1 Departamento de Biofisica e Biometria, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, 20551-030 Rio de Janeiro,2 and Departamento de Genética, Instituto de Biologia, CCS, Universidade Federal do Rio de Janeiro, 21944-970 Rio de Janeiro,3 Brazil, and Department of Microbiology and Immunology, Tulane University Medical Center, School of Medicine, New Orleans, Louisiana 701124

Received 28 December 1999/Returned for modification 10 February 2000/Accepted 4 April 2000

Systemic and mucosal antibody responses against both the major subunit of colonization factor antigen I (CFA/I) of enterotoxigenic Escherichia coli (ETEC) and the somatic lipopolysaccharide expressed by recombinant bivalent Salmonella vaccine strains were significantly enhanced by coadministration of a detoxified derivative with preserved adjuvant effects of the ETEC heat-labile toxin, LT(R192G). The results further support the adjuvant effects of LT(R192G) and represent a simple alternative to improve responses against passenger antigens expressed by orally delivered Salmonella vaccine strains.

* Corresponding author. Present address: Departamento de Microbiologia, Universidade de São Paulo, Institute of Biomedical Sciences-ICB II, Av. Prof. Lineu Prestes, 1374-1st Fl. São Paulo, 05508-900, Brazil. Phone: 5521-560-8093. Fax: 5521-280-8193. E-mail: lcsf{at}biof.ufrj.br.

Infection and Immunity, July 2000, p. 4349-4353, Vol. 68, No. 7
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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