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Infection and Immunity, August 2000, p. 4574-4577, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

CD4+ Cells Are Indispensable for Ulcer Development in Murine Cutaneous Leishmaniasis

Masaki Terabe,1,dagger Takashi Kuramochi,2,3 Mamoru Ito,2,3 Toshimitsu Hatabu,1 Chizu Sanjoba,1 Kwang-Poo Chang,4 Takashi Onodera,1 and Yoshitsugu Matsumoto1,*

Department of Molecular Immunology, School of Agriculture and Life Sciences, University of Tokyo, Tokyo 113,1 Laboratory of Immunology, Central Institute for Experimental Animals, Kawasaki, Kanagawa 216,2 and 8th Laboratory, Kanagawa Academy of Science and Technology, Kawasaki, Kanagawa 213,3 Japan, and Department of Microbiology/Immunology, University of Health Science, Chicago Medical School, North Chicago, Illinois 600644

Received 2 September 1999/Returned for modification 28 October 1999/Accepted 12 May 2000

One of the most characteristic clinical features in cutaneous leishmaniasis is the development of nodules followed by ulcerations at the site of infection. Leishmania amazonensis-infected mice show similar ulcerative lesions. Leishmania-infected severe combined immunodeficiency (SCID) mice, however, have been shown to develop nonulcerative nodules. In the present study, the roles of T cells in ulceration were examined using SCID mice in cell reconstitution experiments. After development of nonulcerative nodules, SCID mice were inoculated with splenocytes from either Leishmania-infected or naive immunocompetent mice, resulting in ulceration in all mice. When naive splenocytes were depleted of CD4+, CD8+, or B220+ cell populations and the remaining cells were injected into Leishmania-infected SCID mice after the development of nodules, only SCID mice inoculated with splenocytes depleted of CD4+ cells did not show ulceration. The evidence obtained in this study clearly shows that the CD4+ cell population is indispensable for ulceration in leishmaniasis lesions of SCID mice.

* Corresponding author. Mailing address: Department of Molecular Immunology, School of Agriculture and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Phone: 81-3-5841-5197. Fax: 81-3-5841-8020. E-mail: aysmatsu{at}mail.ecc.u-tokyo.ac.jp.

dagger Present address: Molecular Immunogenetics and Vaccine Research Section, Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

Infection and Immunity, August 2000, p. 4574-4577, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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