This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tong, H. H.
Right arrow Articles by DeMaria, T. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tong, H. H.
Right arrow Articles by DeMaria, T. F.

 Previous Article  |  Next Article 

Infection and Immunity, August 2000, p. 4593-4597, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of Phase Variation of Nontypeable Haemophilus influenzae Lipooligosaccharide during Nasopharyngeal Colonization and Development of Otitis Media in the Chinchilla Model

H. H. Tong, L. E. Blue, M. A. James, Y. P. Chen, and T. F. DeMaria*

Division of Otologic Research, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio 43210

Received 10 March 2000/Returned for modification 26 April 2000/Accepted 11 May 2000

Nontypeable Haemophilus influenzae (NTHI) has four loci, lic-1 to lic-3 and lgtC, that generate phase-variable lipooligosaccharide (LOS) structures. lic-1, which is required for the expression of phosphorylcholine (ChoP), is the best characterized and is associated with an enhanced ability of H. influenzae to persist within the nasopharynges of infant rats. Recent data indicate that LOS impacts various aspects of NTHI virulence in the chinchilla model of nasopharyngeal colonization and otitis media (OM). In this study the effects of ChoP expression and the sequences of lic-1 to lic-3 and lgtC of NTHI strain 2019 were evaluated in the chinchilla OM model. Nasopharyngeal colonization data showed that a switch from the ChoP- to the ChoP+ phenotype was observed as early as day 3 after intranasal inoculation. Chinchillas colonized by strains with the ChoP+ phenotype demonstrated a significantly higher level of NTHI 2019 per milliliter of nasal lavage fluid than chinchillas colonized with predominantly the ChoP- variant (P < 0.05). The concentration of cells with the ChoP+ phenotype in the middle ear was 3 log units higher than that of cells with the ChoP- variant (P < 0.01). There was a statistically significant association between ChoP+ expression in the nasal lavage and the development of OM with culture-positive middle ear fluids in this model. These data suggest that expression of the ChoP+ phenotype promotes enhanced nasopharyngeal colonization and development of OM.

* Corresponding author. Mailing address: Division of Otologic Research, The Ohio State University, College of Medicine and Public Health, Room 4331 UHC, 456 W. 10th Ave., Columbus, OH 43210. Phone: (614) 293-8103. Fax: (614) 293-5506. E-mail: demaria.2{at}osu.edu.

Infection and Immunity, August 2000, p. 4593-4597, Vol. 68, No. 8
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Armbruster, C. E., Hong, W., Pang, B., Dew, K. E., Juneau, R. A., Byrd, M. S., Love, C. F., Kock, N. D., Swords, W. E. (2009). LuxS Promotes Biofilm Maturation and Persistence of Nontypeable Haemophilus influenzae In Vivo via Modulation of Lipooligosaccharides on the Bacterial Surface. Infect. Immun. 77: 4081-4091 [Abstract] [Full Text]  
  • Toropainen, M., Raitolehto, A., Henckaerts, I., Wauters, D., Poolman, J., Lestrate, P., Kayhty, H. (2008). Pneumococcal Haemophilus influenzae Protein D Conjugate Vaccine Induces Antibodies That Inhibit Glycerophosphodiester Phosphodiesterase Activity of Protein D. Infect. Immun. 76: 4546-4553 [Abstract] [Full Text]  
  • Pang, B., Winn, D., Johnson, R., Hong, W., West-Barnette, S., Kock, N., Swords, W. E. (2008). Lipooligosaccharides Containing Phosphorylcholine Delay Pulmonary Clearance of Nontypeable Haemophilus influenzae. Infect. Immun. 76: 2037-2043 [Abstract] [Full Text]  
  • McCrea, K. W., Xie, J., LaCross, N., Patel, M., Mukundan, D., Murphy, T. F., Marrs, C. F., Gilsdorf, J. R. (2008). Relationships of Nontypeable Haemophilus influenzae Strains to Hemolytic and Nonhemolytic Haemophilus haemolyticus Strains. J. Clin. Microbiol. 46: 406-416 [Abstract] [Full Text]  
  • Hong, W., Mason, K., Jurcisek, J., Novotny, L., Bakaletz, L. O., Swords, W. E. (2007). Phosphorylcholine Decreases Early Inflammation and Promotes the Establishment of Stable Biofilm Communities of Nontypeable Haemophilus influenzae Strain 86-028NP in a Chinchilla Model of Otitis Media. Infect. Immun. 75: 958-965 [Abstract] [Full Text]  
  • West-Barnette, S., Rockel, A., Swords, W. E. (2006). Biofilm Growth Increases Phosphorylcholine Content and Decreases Potency of Nontypeable Haemophilus influenzae Endotoxins. Infect. Immun. 74: 1828-1836 [Abstract] [Full Text]  
  • King, S. J., Whatmore, A. M., Dowson, C. G. (2005). NanA, a Neuraminidase from Streptococcus pneumoniae, Shows High Levels of Sequence Diversity, at Least in Part through Recombination with Streptococcus oralis. J. Bacteriol. 187: 5376-5386 [Abstract] [Full Text]  
  • Swords, W. E., Moore, M. L., Godzicki, L., Bukofzer, G., Mitten, M. J., VonCannon, J. (2004). Sialylation of Lipooligosaccharides Promotes Biofilm Formation by Nontypeable Haemophilus influenzae. Infect. Immun. 72: 106-113 [Abstract] [Full Text]  
  • Warren, M. J., Jennings, M. P. (2003). Identification and Characterization of pptA: a Gene Involved in the Phase-Variable Expression of Phosphorylcholine on Pili of Neisseria meningitidis. Infect. Immun. 71: 6892-6898 [Abstract] [Full Text]  
  • Mason, K. M., Munson, R. S. Jr., Bakaletz, L. O. (2003). Nontypeable Haemophilus influenzae Gene Expression Induced In Vivo in a Chinchilla Model of Otitis Media. Infect. Immun. 71: 3454-3462 [Abstract] [Full Text]  
  • Swords, W. E., Jones, P. A., Apicella, M. A. (2003). Review: The lipo-oligosaccharides of Haemophilus influenzae: an interesting array of characters. Innate Immunity 9: 131-144 [Abstract]  
  • Starner, T. D., Swords, W. E., Apicella, M. A., McCray, P. B. Jr. (2002). Susceptibility of Nontypeable Haemophilus influenzae to Human {beta}-Defensins Is Influenced by Lipooligosaccharide Acylation. Infect. Immun. 70: 5287-5289 [Abstract] [Full Text]  
  • Swords, W. E., Chance, D. L., Cohn, L. A., Shao, J., Apicella, M. A., Smith, A. L. (2002). Acylation of the Lipooligosaccharide of Haemophilus influenzae and Colonization: an htrB Mutation Diminishes the Colonization of Human Airway Epithelial Cells. Infect. Immun. 70: 4661-4668 [Abstract] [Full Text]  
  • Pettigrew, M. M., Foxman, B., Marrs, C. F., Gilsdorf, J. R. (2002). Identification of the Lipooligosaccharide Biosynthesis Gene lic2B as a Putative Virulence Factor in Strains of Nontypeable Haemophilus influenzae That Cause Otitis Media. Infect. Immun. 70: 3551-3556 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»