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Infection and Immunity, September 2000, p. 4968-4971, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mucosal Defense against Gastrointestinal Nematodes: Responses of Mucosal Mast Cells and Mouse Mast Cell Protease 1 during Primary Strongyloides venezuelensis Infection in FcRgamma -Knockout Mice

Denis N. Onah,1,* Fukumi Uchiyama,1 Yuuko Nagakui,1 Masao Ono,2,3 Toshiyuki Takai,2,3 and Yukifumi Nawa1

Department of Parasitology, Miyazaki Medical College, Miyazaki 889-1692,1 Department of Experimental Immunology, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575,2 and CREST, Japan Science and Technology Corporation, Tokyo 101-0062,3 Japan

Received 1 February 2000/Returned for modification 23 March 2000/Accepted 12 June 2000

A possible role for the gamma  subunit of immunoglobulin Fc receptors (FcR) in mucosal defenses against intestinal nematode parasites was studied using age-matched FcRgamma -knockout (FcRgamma -/-) and wild-type (FcRgamma +/+) C57BL/6 mice. Mice were infected subcutaneously with 3,000 infective larvae of Strongyloides venezuelensis, and the degree of infection was monitored by daily fecal egg counts and adult worm recovery on days 8 and 13 postinfection. Mucosal mast cell (MMC) responses were assayed by in situ intestinal mast cell counts in stained histological sections of the jejunum and by measuring mouse mast cell protease 1 (MMCP-1) release in serum using sandwich enzyme-linked immunosorbent assay. FcRgamma -/- mice had significantly higher egg counts (P < 0.01) and numbers of adult worms (P < 0.05) than FcRgamma +/+ mice, but mastocytosis and serum MMCP-1 release were comparable. It was concluded that MMCP-1 release may be spontaneous, does not depend on mast cell degranulation via the FcRgamma signaling system, and appears to play no role in the expulsion of S. venezuelensis. The delay in worm expulsion in the FcRgamma -/- mice might be related to inability of the MMC to degranulate and release effector molecules other than MMCP-1, since FcRgamma deletion abrogates mast cell degranulative responses.

* Corresponding author. Mailing address: Department of Parasitology, Miyazaki Medical College, Kiyotake, Miyazaki 889-1692, Japan. Phone: 81-985-85-0990. Fax: 81-984-84-3887. E-mail: denis{at}fc.miyazaki-med.ac.jp.

Infection and Immunity, September 2000, p. 4968-4971, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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