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Infection and Immunity, September 2000, p. 5401-5404, Vol. 68, No. 9
Biotechnology Laboratory, Department of Biochemistry and
Molecular Biology, and Department of Microbiology and Immunology,
University of British Columbia, Vancouver, British Columbia V6T
1Z3,1 and Infectious Diseases Unit,
CHUL, Laval University, Quebec G1V 4G2,2 Canada
Received 25 February 2000/Returned for modification 26 April
2000/Accepted 8 June 2000
Phagosome acidification is an important component
of the microbicidal response by infected eukaryotic cells. Thus,
intracellular pathogens that reside within phagosomes must either block
phagosome acidification or be able to survive at low pH. In this work,
we studied the effect of phagosomal acidification on the survival of
intracellular Salmonella enterica serovar Typhimurium in
different cell types. Bafilomycin A1, a specific inhibitor of the
vacuolar proton-ATPases, was used to block acidification of
salmonella-containing vacuoles. We found that in several
epithelial cell lines, treatment with bafilomycin A1 had no effect
on intracellular survival or replication. Furthermore, although
acidification was essential for Salmonella intracellular
survival in J774 cultured macrophages, as reported previously
(13), it is not essential in other macrophage cell lines.
These data suggest that vacuolar acidification may play a role in
intracellular survival of salmonellae only under certain conditions and
in specific cell types.
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Vacuole Acidification Is Not Required for Survival of
Salmonella enterica Serovar Typhimurium within Cultured
Macrophages and Epithelial Cells
*
Corresponding author. Mailing address: Biotechnology
Laboratory, University of British Columbia, 237-6174 University
Boulevard, Vancouver, BC V6T 1Z3, Canada. Phone: (604) 822-2110. Fax:
(604) 822-9830. E-mail: bfinlay{at}unixg.ubc.ca.
Present address: Department of Cell Biology & Physiology,
Washington University School of Medicine, St. Louis, MO 63110.
Present address: Hema-Quebec, Ste-Foy, Qc G1V 4M3, Canada.
Infection and Immunity, September 2000, p. 5401-5404, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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