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Infection and Immunity, September 2000, p. 5416-5419, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Invasion of Human Vascular Endothelial Cells by Actinobacillus actinomycetemcomitans via the Receptor for Platelet-Activating Factor

Harvey A. Schenkein,* Suzanne E. Barbour, C. R. Berry, Barbara Kipps, and John G. Tew

Clinical Research Center for Periodontal Diseases, School of Dentistry, Virginia Commonwealth University, Richmond, Virginia 23298

Received 31 January 2000/Returned for modification 15 March 2000/Accepted 30 May 2000

Strains of the periodontal pathogen Actinobacillus actinomycetemcomitans are variable with respect to display of phosphorylcholine (PC)-bearing antigens. We have examined strains of A. actinomycetemcomitans with and without PC to assess their ability to invade endothelial cells via the receptor for platelet-activating factor (PAF). Results of antibiotic protection assays indicate that PC-bearing A. actinomycetemcomitans invade human vascular endothelial cells by a mechanism inhibitable by CV3988, a PAF receptor antagonist, and by PAF itself. The invasive phenotype was verified by transmission electron microscopy. A PC-deficient strain of this organism was not invasive. This property, in addition to the established ability of A. actinomycetemcomitans to invade epithelial cells, may provide this organism with access to the systemic circulation. The ability of PC-bearing oral bacteria to access the circulation may also explain the elevated levels of anti-PC antibody in serum found in patients with periodontitis.

* Corresponding author. Mailing address: VCU School of Dentistry, P.O. Box 980566, Richmond, VA 23298-0566. Phone: (804) 828-9185. Fax: (804) 828-5787. E-mail: hschenke{at}hsc.vcu.edu.

Infection and Immunity, September 2000, p. 5416-5419, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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