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Infection and Immunity, September 2000, p. 5450-5453, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Random Transposon Mutagenesis of Campylobacter jejuni

Neal J. Golden,1 Andrew Camilli,2 and David W. K. Acheson1,3,*

Division of Geographic Medicine and Infectious Diseases, New England Medical Center,3 and Departments of Immunology and Pathology1 and Molecular Microbiology,2 Tufts University, Boston, Massachusetts 02111

Received 3 February 2000/Returned for modification 22 March 2000/Accepted 14 June 2000

Genetic studies of Campylobacter jejuni have been limited due to the lack of a transposon mutagenesis method. Here, we describe a novel technique for random transposon mutagenesis using a mariner-based transposon into C. jejuni strain 480. Insertions were random, as demonstrated by Southern blot analysis and insertional junction sequencing. We have demonstrated, for the first time, random in vivo transposon mutagenesis of C. jejuni.

* Corresponding author. Mailing address: Division of Geographic Medicine and Infectious Diseases, New England Medical Center, Box 041, 750 Washington St., Boston, MA 02111. Phone: (617) 636-7002. Fax: (617) 636-5292. E-mail: dacheson{at}lifespan.org.

Infection and Immunity, September 2000, p. 5450-5453, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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