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Infection and Immunity, September 2000, p. 5466-5468, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Presence of Phosphorylcholine on a Filarial Nematode Protein Influences Immunoglobulin G Subclass Response to the Molecule by an Interleukin-10-Dependent Mechanism

Katrina M. Houston,1 Emma H. Wilson,1 Linda Eyres,1,dagger Frank Brombacher,2 Margaret M. Harnett,3 James Alexander,1 and William Harnett1,*

Department of Immunology, University of Strathclyde, Glasgow G4 0NR,1 and Department of Immunology, University of Glasgow, Glasgow G11 6NT,3 United Kingdom, and Department of Immunology, University of Cape Town, 7925 Cape Town, South Africa2

Received 15 February 2000/Returned for modification 28 March 2000/Accepted 23 June 2000

The filarial nematode product ES-62 contains phosphorylcholine (PC) covalently attached to N-linked glycans. ES-62 induced high levels of immunoglobulin G1 (IgG1) antibodies, but no IgG2a, to non-PC epitopes of the molecule following subcutaneous injection into BALB/c mice. Conversely, mice given ES-62 lacking PC demonstrated significant production of both IgG subclasses. Thus, PC appears to block production of IgG2a antibodies to other epitopes on the parasite molecule. A role for interleukin-10 (IL-10) in this effect was shown by the ability of IL-10-/- mice to make an IgG2a antibody response to non-PC epitopes of ES-62.

* Corresponding author. Mailing address: Department of Immunology, The Todd Wing, Strathclyde Institute for Biomedical Sciences, 27 Taylor St., Glasgow G4 0NR, United Kingdom. Phone: 44-141-5483725. Fax: 44-141-5526674. E-mail: w.harnett{at}strath.ac.uk.

dagger Present address: Division of Biochemistry, School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom.

Infection and Immunity, September 2000, p. 5466-5468, Vol. 68, No. 9
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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