This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jersmann, H. P. A.
Right arrow Articles by Ferrante, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jersmann, H. P. A.
Right arrow Articles by Ferrante, A.

 Previous Article  |  Next Article 

Infection and Immunity, January 2001, p. 479-485, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.479-485.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Synthesis and Surface Expression of CD14 by Human Endothelial Cells

Hubertus P. A. Jersmann,1 Charles S. T. Hii,1 Greg L. Hodge,2 and Antonio Ferrante1,*

Department of Immunopathology1 and Department of Haematology,2 Women's and Children's Hospital, North Adelaide, South Australia 5006, Australia

Received 11 February 2000/Returned for modification 15 May 2000/Accepted 18 October 2000

Previous studies have reported that human vascular endothelial cells lack the membrane-bound lipopolysaccharide (LPS) receptor, CD14 (mCD14). By optimizing assay conditions, including the selection of anti-CD14 monoclonal antibody, we now demonstrate that human umbilical vein endothelial cells (HUVEC) express CD14 on the cell surface. Single-passage HUVEC showed approximately 20 times less expression of CD14 than monocytes. Interestingly, there was significant loss of surface CD14 expression with increasing numbers of culture passages. Evidence for synthesis of CD14 by HUVEC was provided by the finding that L-[35S]methionine was incorporated into CD14. In addition, the expression of CD14 on HUVEC was upregulated by LPS, lysophosphatidic acid, and tissue culture supplements, and this upregulation was dependent on protein synthesis. Furthermore, the results imply that mCD14 is required for LPS-induced activation of endothelial cells in the absence of serum and that it acts in concert with serum factors (soluble CD14). Our results provide evidence that CD14 is expressed by endothelial cells and suggest that the previous inability to observe expression of this molecule has been due to culture and staining conditions. This finding has important implications for the understanding of the mechanisms by which LPS stimulates endothelial cells and the management of sepsis caused by gram-negative bacteria.

* Corresponding author. Mailing address: Department of Immunopathology, Women's and Children's Hospital, 72 King William Rd., North Adelaide, South Australia 5006, Australia. Phone: 61-8-82047216. Fax: 61-8-82046046. E-mail: aferrant{at}medicine.adelaide.edu.au.

Infection and Immunity, January 2001, p. 479-485, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.479-485.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Mills, N L, Tura, O, Padfield, G J, Millar, C, Lang, N N, Stirling, D, Ludlam, C, Turner, M L, Barclay, G R, Newby, D E (2009). Dissociation of phenotypic and functional endothelial progenitor cells in patients undergoing percutaneous coronary intervention. Heart 95: 2003-2008 [Abstract] [Full Text]  
  • Lloyd-Jones, K. L., Kelly, M. M., Kubes, P. (2008). Varying Importance of Soluble and Membrane CD14 in Endothelial Detection of Lipopolysaccharide. J. Immunol. 181: 1446-1453 [Abstract] [Full Text]  
  • Gong, P., Angelini, D. J., Yang, S., Xia, G., Cross, A. S., Mann, D., Bannerman, D. D., Vogel, S. N., Goldblum, S. E. (2008). TLR4 Signaling Is Coupled to SRC Family Kinase Activation, Tyrosine Phosphorylation of Zonula Adherens Proteins, and Opening of the Paracellular Pathway in Human Lung Microvascular Endothelia. J. Biol. Chem. 283: 13437-13449 [Abstract] [Full Text]  
  • Teghanemt, A., Prohinar, P., Gioannini, T. L., Weiss, J. P. (2007). Transfer of Monomeric Endotoxin from MD-2 to CD14: CHARACTERIZATION AND FUNCTIONAL CONSEQUENCES. J. Biol. Chem. 282: 36250-36256 [Abstract] [Full Text]  
  • Dominguez-Punaro, M. C., Segura, M., Plante, M.-M., Lacouture, S., Rivest, S., Gottschalk, M. (2007). Streptococcus suis Serotype 2, an Important Swine and Human Pathogen, Induces Strong Systemic and Cerebral Inflammatory Responses in a Mouse Model of Infection. J. Immunol. 179: 1842-1854 [Abstract] [Full Text]  
  • Robb, A. O, Mills, N. L, Newby, D. E, Denison, F. C (2007). Endothelial progenitor cells in pregnancy. Reproduction 133: 1-9 [Abstract] [Full Text]  
  • Lloyd, K. L., Kubes, P. (2006). GPI-linked endothelial CD14 contributes to the detection of LPS. Am. J. Physiol. Heart Circ. Physiol. 291: H473-H481 [Abstract] [Full Text]  
  • Regueiro, V., Campos, M. A., Pons, J., Alberti, S., Bengoechea, J. A. (2006). The uptake of a Klebsiella pneumoniae capsule polysaccharide mutant triggers an inflammatory response by human airway epithelial cells. Microbiology 152: 555-566 [Abstract] [Full Text]  
  • Yang, X., Coriolan, D., Murthy, V., Schultz, K., Golenbock, D. T., Beasley, D. (2005). Proinflammatory phenotype of vascular smooth muscle cells: role of efficient Toll-like receptor 4 signaling. Am. J. Physiol. Heart Circ. Physiol. 289: H1069-H1076 [Abstract] [Full Text]  
  • Stoll, L. L., Denning, G. M., Weintraub, N. L. (2004). Potential Role of Endotoxin as a Proinflammatory Mediator of Atherosclerosis. Arterioscler. Thromb. Vasc. Bio. 24: 2227-2236 [Abstract] [Full Text]  
  • Shimizu, T., Yokota, S.-i., Takahashi, S., Kunishima, Y., Takeyama, K., Masumori, N., Takahashi, A., Matsukawa, M., Itoh, N., Tsukamoto, T., Fujii, N. (2004). Membrane-Anchored CD14 Is Important for Induction of Interleukin-8 by Lipopolysaccharide and Peptidoglycan in Uroepithelial Cells. CVI 11: 969-976 [Abstract] [Full Text]  
  • Dunzendorfer, S., Lee, H.-K., Soldau, K., Tobias, P. S. (2004). TLR4 Is the Signaling but Not the Lipopolysaccharide Uptake Receptor. J. Immunol. 173: 1166-1170 [Abstract] [Full Text]  
  • Stoll, L. L., Denning, G. M., Li, W.-G., Rice, J. B., Harrelson, A. L., Romig, S. A., Gunnlaugsson, S. T., Miller, F. J. Jr, Weintraub, N. L. (2004). Regulation of Endotoxin-Induced Proinflammatory Activation in Human Coronary Artery Cells: Expression of Functional Membrane-Bound CD14 by Human Coronary Artery Smooth Muscle Cells. J. Immunol. 173: 1336-1343 [Abstract] [Full Text]  
  • Gioannini, T. L., Teghanemt, A., Zarember, K. A., Weiss, J. P. (2003). Regulation of interactions of endotoxin with host cells. Innate Immunity 9: 401-408 [Abstract]  
  • Coats, S. R., Reife, R. A., Bainbridge, B. W., Pham, T.-T. T., Darveau, R. P. (2003). Porphyromonas gingivalis Lipopolysaccharide Antagonizes Escherichia coli Lipopolysaccharide at Toll-Like Receptor 4 in Human Endothelial Cells. Infect. Immun. 71: 6799-6807 [Abstract] [Full Text]  
  • Peters, K., Unger, R. E., Brunner, J., Kirkpatrick, C.J. (2003). Molecular basis of endothelial dysfunction in sepsis. Cardiovasc Res 60: 49-57 [Abstract] [Full Text]  
  • Walton, K. A., Hsieh, X., Gharavi, N., Wang, S., Wang, G., Yeh, M., Cole, A. L., Berliner, J. A. (2003). Receptors Involved in the Oxidized 1-Palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine-mediated Synthesis of Interleukin-8: A ROLE FOR TOLL-LIKE RECEPTOR 4 AND A GLYCOSYLPHOSPHATIDYLINOSITOL-ANCHORED PROTEIN. J. Biol. Chem. 278: 29661-29666 [Abstract] [Full Text]  
  • Walton, K. A., Cole, A. L., Yeh, M., Subbanagounder, G., Krutzik, S. R., Modlin, R. L., Lucas, R. M., Nakai, J., Smart, E. J., Vora, D. K., Berliner, J. A. (2003). Specific Phospholipid Oxidation Products Inhibit Ligand Activation of Toll-Like Receptors 4 and 2. Arterioscler. Thromb. Vasc. Bio. 23: 1197-1203 [Abstract] [Full Text]  
  • Schilling, J. D., Martin, S. M., Hunstad, D. A., Patel, K. P., Mulvey, M. A., Justice, S. S., Lorenz, R. G., Hultgren, S. J. (2003). CD14- and Toll-Like Receptor-Dependent Activation of Bladder Epithelial Cells by Lipopolysaccharide and Type 1 Piliated Escherichia coli. Infect. Immun. 71: 1470-1480 [Abstract] [Full Text]  
  • Zeuke, S., Ulmer, A. J, Kusumoto, S., Katus, H. A, Heine, H. (2002). TLR4-mediated inflammatory activation of human coronary artery endothelial cells by LPS. Cardiovasc Res 56: 126-134 [Abstract] [Full Text]  
  • Davidson, K. G., Bersten, A. D., Barr, H. A., Dowling, K. D., Nicholas, T. E., Doyle, I. R. (2002). Endotoxin Induces Respiratory Failure and Increases Surfactant Turnover and Respiration Independent of Alveolocapillary Injury in Rats. Am. J. Respir. Crit. Care Med. 165: 1516-1525 [Abstract] [Full Text]  
  • Jersmann, H. P. A., Hii, C. S. T., Ferrante, J. V., Ferrante, A. (2001). Bacterial Lipopolysaccharide and Tumor Necrosis Factor Alpha Synergistically Increase Expression of Human Endothelial Adhesion Molecules through Activation of NF-{kappa}B and p38 Mitogen-Activated Protein Kinase Signaling Pathways. Infect. Immun. 69: 1273-1279 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»