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Infection and Immunity, January 2001, p. 559-563, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.559-563.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Tir Tyrosine Phosphorylation and Pedestal Formation Are Delayed in Enteropathogenic Escherichia coli sepZ::TnphoA Mutant 30-5-1(3)

Rebekah Devinney,1,dagger Israel Nisan,2,3 Sharon Ruschkowski,1 Ilan Rosenshine,2 and B. Brett Finlay1,*

Biotechnology Laboratory, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada,1 and Departments of Molecular Genetics and Biotechnology2 and Clinical Microbiology,3 Hebrew University, Jerusalem, 91120, Israel

Received 11 April 2000/Returned for modification 20 June 2000/Accepted 16 October 2000

Enteropathogenic Escherichia coli (EPEC) strain 30-5-1(3) has been reported to form attaching and effacing (A/E) lesions without Tir tyrosine phosphorylation. In this study, we show that 30-5-1(3), which has a transposon insertion within the sepZ gene, forms wild-type A/E lesions including Tir tyrosine phosphorylation, but at a slower rate. A/E lesion formation by 30-5-1(3) occurs without detectable secretion of Tir or other EPEC Esp secreted proteins.

* Corresponding author. Mailing address: Biotechnology Laboratory, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada. Phone: (604) 822-2210. Fax: (604) 822-9830. E-mail: bfinlay{at}unixg.ubc.ca.

dagger Present address: Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada.

Infection and Immunity, January 2001, p. 559-563, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.559-563.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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