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Infection and Immunity, January 2001, p. 602-606, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.602-606.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Effect of Influenza A Virus Infection on Nasopharyngeal Colonization and Otitis Media Induced by Transparent or Opaque Phenotype Variants of Streptococcus pneumoniae in the Chinchilla Model

H. H. Tong,1 J. N. Weiser,2 M. A. James,1 and T. F. DeMaria1,*

Division of Otologic Research, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio 43210,1 and Departments of Pediatrics and Microbiology, Children's Hospital of Philadelphia and University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 191042

Received 21 July 2000/Returned for modification 28 September 2000/Accepted 13 October 2000

Phase variation in the colonial opacity of Streptococcus pneumoniae has been implicated as a factor in bacterial adherence, colonization, and invasion in the pathogenesis of pneumococcal disease. Additionally, the synergistic effects of influenza A virus and S. pneumoniae in the development of otitis media (OM) have been reported. This study examined the ability of opaque or transparent S. pneumoniae from the same strain in combination with an antecedent influenza A virus infection to colonize the nasopharynx and invade the middle ear in the chinchilla model. Our data indicated that there was no significant difference in the level of nasopharyngeal colonization and induction of OM between the opaque and transparent variants unless there was a prior challenge with influenza A virus. Subsequent to influenza A virus infection, there was a significant difference between the variants in the ability to colonize and persist in the nasopharynx and middle ear. The concentrations of the opaque variant in nasopharyngeal-lavage samples and middle-ear fluid remained consistently higher than those of the transparent variant for 10 days postinoculation. Data from this study indicate that the effects of influenza A virus on the pathogenesis of experimental S. pneumoniae-induced OM differ depending on the opacity phenotype involved.

* Corresponding author. Mailing address: Division of Otologic Research, College of Medicine and Public Health, The Ohio State University, Room 4331 UHC, 456 W 10th Ave., Columbus, OH 43210. Phone: (614) 293-8103. Fax: (614) 293-5506. E-mail: demaria.2{at}osu.edu.

Infection and Immunity, January 2001, p. 602-606, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.602-606.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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