This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smyth, A. J.
Right arrow Articles by Pollock, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smyth, A. J.
Right arrow Articles by Pollock, J. M.

 Previous Article  |  Next Article 

Infection and Immunity, January 2001, p. 89-96, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.89-96.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

In Vitro Responsiveness of gamma delta T Cells from Mycobacterium bovis-Infected Cattle to Mycobacterial Antigens: Predominant Involvement of WC1+ Cells

Allister J. Smyth,1 Michael D. Welsh,2,* R. Martyn Girvin,2 and John M. Pollock2

Department of Veterinary Science, The Queen's University of Belfast,1 and Veterinary Sciences Division, The Department of Agriculture and Rural Development,2 Stormont, Belfast BT4 3SD, United Kingdom

Received 20 March 2000/Returned for modification 15 May 2000/Accepted 27 September 2000

It is generally accepted that protective immunity against tuberculosis is generated through the cell-mediated immune (CMI) system, and a greater understanding of such responses is required if better vaccines and diagnostic tests are to be developed. gamma delta T cells form a major proportion of the peripheral blood mononuclear cells (PBMC) in the ruminant system and, considering data from other species, may have a significant role in CMI responses in bovine tuberculosis. This study compared the in vitro responses of alpha beta and gamma delta T cells from Mycobacterium bovis-infected and uninfected cattle. The results showed that, following 24 h of culture of PBMC with M. bovis-derived antigens, the majority of gamma delta T cells from infected animals became highly activated (upregulation of interleukin-2R), while a lower proportion of the alpha beta T-cell population showed activation. Similar responses were evident to a lesser degree in uninfected animals. Study of the kinetics of this response showed that gamma delta T cells remained significantly activated for at least 7 days in culture, while activation of alpha beta T cells declined during that period. Subsequent analysis revealed that the majority of activated gamma delta T cells expressed WC1, a 215-kDa surface molecule which is not expressed on human or murine gamma delta T cells. Furthermore, in comparison with what was found for CD4+ T cells, M. bovis antigen was found to induce strong cellular proliferation but relatively little gamma interferon release by purified WC1+ gamma delta T cells. Overall, while the role of these cells in protective immunity remains unclear, their highly activated status in response to M. bovis suggests an important role in antimycobacterial immunity, and the ability of gamma delta T cells to influence other immune cell functions remains to be elucidated, particularly in relation to CMI-based diagnostic tests.

* Corresponding author. Mailing address: Department of Bacteriology, Veterinary Sciences Division, The Department of Agriculture and Rural Development, Stoney Road, Stormont, Belfast BT4 3SD, United Kingdom. Phone: 028 90 525642. Fax: 028 90 525745. E-mail: michael.welsh{at}dardni.gov.uk.

Infection and Immunity, January 2001, p. 89-96, Vol. 69, No. 1
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.1.89-96.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ameni, G., Aseffa, A., Engers, H., Young, D., Gordon, S., Hewinson, G., Vordermeier, M. (2007). High Prevalence and Increased Severity of Pathology of Bovine Tuberculosis in Holsteins Compared to Zebu Breeds under Field Cattle Husbandry in Central Ethiopia. CVI 14: 1356-1361 [Abstract] [Full Text]  
  • Liebana, E., Marsh, S., Gough, J., Nunez, A., Vordermeier, H. M., Whelan, A., Spencer, Y., Clifton-Hardley, R., Hewinson, G., Johnson, L. (2007). Distribution and Activation of T-lymphocyte Subsets in Tuberculous Bovine Lymph-node Granulomas. Vet Pathol 44: 366-372 [Abstract] [Full Text]  
  • Van Rhijn, I., Rutten, V. P. M. G., Charleston, B., Smits, M., van Eden, W., Koets, A. P. (2007). Massive, sustained {gamma}{delta} T cell migration from the bovine skin in vivo. J. Leukoc. Biol. 81: 968-973 [Abstract] [Full Text]  
  • Rogers, A. N., VanBuren, D. G., Hedblom, E. E., Tilahun, M. E., Telfer, J. C., Baldwin, C. L. (2005). {gamma}{delta} T Cell Function Varies with the Expressed WC1 Coreceptor. J. Immunol. 174: 3386-3393 [Abstract] [Full Text]  
  • Waters, W. R., Rahner, T. E., Palmer, M. V., Cheng, D., Nonnecke, B. J., Whipple, D. L. (2003). Expression of L-Selectin (CD62L), CD44, and CD25 on Activated Bovine T Cells. Infect. Immun. 71: 317-326 [Abstract] [Full Text]  
  • Welsh, M. D., Kennedy, H. E., Smyth, A. J., Girvin, R. M., Andersen, P., Pollock, J. M. (2002). Responses of Bovine WC1+{gamma}{delta} T Cells to Protein and Nonprotein Antigens of Mycobacterium bovis. Infect. Immun. 70: 6114-6120 [Abstract] [Full Text]  
  • Naiman, B. M., Blumerman, S., Alt, D., Bolin, C. A., Brown, R., Zuerner, R., Baldwin, C. L. (2002). Evaluation of Type 1 Immune Response in Naive and Vaccinated Animals following Challenge with Leptospira borgpetersenii Serovar Hardjo: Involvement of WC1+{gamma}{delta} and CD4 T Cells. Infect. Immun. 70: 6147-6157 [Abstract] [Full Text]  
  • Jungersen, G., Huda, A., Hansen, J. J., Lind, P. (2002). Interpretation of the Gamma Interferon Test for Diagnosis of Subclinical Paratuberculosis in Cattle. CVI 9: 453-460 [Abstract] [Full Text]  
  • Kennedy, H. E., Welsh, M. D., Bryson, D. G., Cassidy, J. P., Forster, F. I., Howard, C. J., Collins, R. A., Pollock, J. M. (2002). Modulation of Immune Responses to Mycobacterium bovis in Cattle Depleted of WC1+{gamma}{delta} T Cells. Infect. Immun. 70: 1488-1500 [Abstract] [Full Text]  
  • Naiman, B. M., Alt, D., Bolin, C. A., Zuerner, R., Baldwin, C. L. (2001). Protective Killed Leptospira borgpetersenii Vaccine Induces Potent Th1 Immunity Comprising Responses by CD4 and gamma delta T Lymphocytes. Infect. Immun. 69: 7550-7558 [Abstract] [Full Text]  
  • Waters, W. R., Nonnecke, B. J., Rahner, T. E., Palmer, M. V., Whipple, D. L., Horst, R. L. (2001). Modulation of Mycobacterium bovis-Specific Responses of Bovine Peripheral Blood Mononuclear Cells by 1,25-Dihydroxyvitamin D3. CVI 8: 1204-1212 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»