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Infection and Immunity, October 2001, p. 6148-6155, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6148-6155.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Shiga Toxin Translocation across Intestinal Epithelial Cells Is Enhanced by Neutrophil Transmigration

Bryan P. Hurley,1,2 Cheleste M. Thorpe,1 and David W. K. Acheson1,2,*

Division of Geographic Medicine and Infectious Disease, New England Medical Center,1 and Department of Pathology, Tufts University,2 Boston, Massachusetts 02111

Received 23 March 2001/Returned for modification 25 April 2001/Accepted 22 June 2001

Shiga toxin-producing E. coli (STEC) is a food-borne pathogen that causes serious illness, including hemolytic-uremic syndrome (HUS). STEC colonizes the lower intestine and produces Shiga toxins (Stxs). Stxs appear to translocate across intestinal epithelia and affect sensitive endothelial cell beds at various sites. We have previously shown that Stxs cross polarized intestinal epithelial cells (IECs) via a transcellular route and remain biologically active. Since acute inflammatory infiltration of the gut and fecal leukocytes is seen in many STEC-infected patients and since polymorphonuclear leukocyte (PMN) transmigration across polarized IECs diminishes the IEC barrier function in vitro, we hypothesized that PMN transmigration may enhance Stx movement across IECs. We found that basolateral-to-apical transmigration of neutrophils significantly increased the movement of Stx1 and Stx2 across polarized T84 IECs in the opposite direction. The amount of Stx crossing the T84 barrier was proportional to the degree of neutrophil transmigration, and the increase in Stx translocation appears to be due to increases in paracellular permeability caused by migrating PMNs. STEC clinical isolates applied apically induced PMN transmigration across and interleukin-8 (IL-8) secretion from T84 cells. Of the 10 STEC strains tested, three STEC strains lacking eae and espB (eae- and espB-negative STEC strains) induced significantly more neutrophil transmigration and significantly greater IL-8 secretion than eae- and espB-positive STEC or enteropathogenic E. coli. This study suggests that STEC interaction with intestinal epithelia induces neutrophil recruitment to the intestinal lumen, resulting in neutrophil extravasation across IECs, and that during this process Stxs may pass in greater amounts into underlying tissues, thereby increasing the risk of HUS.

* Corresponding author. Mailing address: Department of Epidemiology and Preventive Medicine, University of Maryland, 10 South Pine St., MSTF Building, Room 9-34B, Baltimore, MD 21201. Phone: (410) 706-4583. Fax: (410) 706-4581. E-mail: dacheson{at}epi.umaryland.edu.

Infection and Immunity, October 2001, p. 6148-6155, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6148-6155.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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