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Infection and Immunity, October 2001, p. 6179-6185, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6179-6185.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

TonB-Dependent Systems of Uropathogenic Escherichia coli: Aerobactin and Heme Transport and TonB Are Required for Virulence in the Mouse

Alfredo G. Torres,1,dagger Peter Redford,2 Rodney A. Welch,2 and Shelley M. Payne1,*

Department of Microbiology and the Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712,1 and Department of Medical Microbiology and Immunology, University of Wisconsin---Madison, Madison, Wisconsin 537062

Received 24 April 2001/Returned for modification 18 June 2001/Accepted 11 July 2001

The uropathogenic Escherichia coli strain CFT073 has multiple iron acquisition systems, including heme and siderophore transporters. A tonB mutant derivative of CFT073 failed to use heme as an iron source or to utilize the siderophores enterobactin and aerobactin, indicating that transport of these compounds in CFT073 is TonB dependent. The TonB- derivative showed reduced virulence in a mouse model of urinary tract infection. Virulence was restored when the tonB gene was introduced on a plasmid. To determine the importance of the individual TonB-dependent iron transport systems during urinary tract infections, mutants defective in each of the CFT073 high-affinity iron transport systems were constructed and tested in the mouse model. Mouse virulence assays indicated that mutants defective in a single iron transport system were able to infect the kidney when inoculated as a pure culture but were unable to efficiently compete with the wild-type strain in mixed infections. These results indicate a role for TonB-dependent systems in the virulence of uropathogenic E. coli strains.

* Corresponding author. Mailing address: Department of Microbiology and the Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712. Phone: (512) 471-5204. Fax: (512) 471-7088. E-mail: payne{at}mail.utexas.edu.

dagger Present address: Center for Vaccine Development and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201.

Infection and Immunity, October 2001, p. 6179-6185, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6179-6185.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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