This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Al-Mariri, A.
Right arrow Articles by Letesson, J.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Al-Mariri, A.
Right arrow Articles by Letesson, J.-J.

 Previous Article  |  Next Article 

Infection and Immunity, October 2001, p. 6264-6270, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6264-6270.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Induction of Immune Response in BALB/c Mice with a DNA Vaccine Encoding Bacterioferritin or P39 of Brucella spp.

Ayman Al-Mariri,1 Anne Tibor,1 Pascal Mertens,1 Xavier De Bolle,1 Patrick Michel,2 Jacques Godfroid,2 Karl Walravens,2 and Jean-Jacques Letesson1,*

Unité de Recherche en Biologie Moléculaire (URBM), Laboratoire d'Immunologie et de Microbiologie, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur,1 and Centre d'Etude et de Recherche Vétérinaire et Agrochimique (CERVA), B-1180 Brussels,2 Belgium

Received 24 April 2001/Returned for modification 25 May 2001/Accepted 6 July 2001

In this study, we evaluated the ability of DNA vaccines encoding the bacterioferritin (BFR) or P39 proteins of Brucella spp. to induce cellular and humoral immune responses and to protect BALB/c mice against a challenge with B. abortus 544. We constructed eukaryotic expression vectors called pCIBFR and pCIP39, encoding BFR or P39 antigens, respectively, and we verified that these proteins were produced after transfection of COS-7 cells. PCIBFR or pCIP39 was injected intramuscularly three times, at 3-week intervals. pCIP39 induced higher antibody responses than did the DNA vector encoding BFR. Both vectors elicited a T-cell-proliferative response and also induced a strong gamma interferon production upon restimulation with either the specific antigens or Brucella extract. In this report, we also demonstrat that animals immunized with these plasmids elicited a strong and long-lived memory immune response which persisted at least 3 months after the third vaccination. Furthermore, pCIBFR and pCIP39 induced a typical T-helper 1-dominated immune response in mice, as determined by cytokine or immunoglobulin G isotype analysis. The pCIP39 delivered by intramuscular injection (but not the pCIBFR or control vectors) induced a moderate protection in BALB/c mice challenged with B. abortus 544 compared to that observed in positive control mice vaccinated with S19.

* Corresponding author. Mailing address. Unité de Recherche en Biologie Moléculaire (URBM), Laboratoire d'Immunologie et de Microbiologie, Facultés Universitaires Notre-Dame de la Paix, Rue de Bruxelles 61, B-5000 Namur, Belgium. Phone: 32 81 72 44 02. Fax: 32 81 72 42 97. E-mail: Jean-jacques.letesson{at}fundp.ac.be.

Infection and Immunity, October 2001, p. 6264-6270, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6264-6270.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Savitt, A. G., Mena-Taboada, P., Monsalve, G., Benach, J. L. (2009). Francisella tularensis Infection-Derived Monoclonal Antibodies Provide Detection, Protection, and Therapy. CVI 16: 414-422 [Abstract] [Full Text]  
  • Luo, D., Ni, B., Li, P., Shi, W., Zhang, S., Han, Y., Mao, L., He, Y., Wu, Y., Wang, X. (2006). Protective Immunity Elicited by a Divalent DNA Vaccine Encoding Both the L7/L12 and Omp16 Genes of Brucella abortus in BALB/c Mice.. Infect. Immun. 74: 2734-2741 [Abstract] [Full Text]  
  • Yang, X., Hudson, M., Walters, N., Bargatze, R. F., Pascual, D. W. (2005). Selection of Protective Epitopes for Brucella melitensis by DNA Vaccination. Infect. Immun. 73: 7297-7303 [Abstract] [Full Text]  
  • Cassataro, J., Velikovsky, C. A., de la Barrera, S., Estein, S. M., Bruno, L., Bowden, R., Pasquevich, K. A., Fossati, C. A., Giambartolomei, G. H. (2005). A DNA Vaccine Coding for the Brucella Outer Membrane Protein 31 Confers Protection against B. melitensis and B. ovis Infection by Eliciting a Specific Cytotoxic Response. Infect. Immun. 73: 6537-6546 [Abstract] [Full Text]  
  • Onate, A. A., Donoso, G., Moraga-Cid, G., Folch, H., Cespedes, S., Andrews, E. (2005). An RNA Vaccine Based on Recombinant Semliki Forest Virus Particles Expressing the Cu,Zn Superoxide Dismutase Protein of Brucella abortus Induces Protective Immunity in BALB/c Mice. Infect. Immun. 73: 3294-3300 [Abstract] [Full Text]  
  • Munoz-Montesino, C., Andrews, E., Rivers, R., Gonzalez-Smith, A., Moraga-Cid, G., Folch, H., Cespedes, S., Onate, A. A. (2004). Intraspleen Delivery of a DNA Vaccine Coding for Superoxide Dismutase (SOD) of Brucella abortus Induces SOD-Specific CD4+ and CD8+ T Cells. Infect. Immun. 72: 2081-2087 [Abstract] [Full Text]  
  • Velikovsky, C. A., Goldbaum, F. A., Cassataro, J., Estein, S., Bowden, R. A., Bruno, L., Fossati, C. A., Giambartolomei, G. H. (2003). Brucella Lumazine Synthase Elicits a Mixed Th1-Th2 Immune Response and Reduces Infection in Mice Challenged with Brucella abortus 544 Independently of the Adjuvant Formulation Used. Infect. Immun. 71: 5750-5755 [Abstract] [Full Text]  
  • Onate, A. A., Cespedes, S., Cabrera, A., Rivers, R., Gonzalez, A., Munoz, C., Folch, H., Andrews, E. (2003). A DNA Vaccine Encoding Cu,Zn Superoxide Dismutase of Brucella abortus Induces Protective Immunity in BALB/c Mice. Infect. Immun. 71: 4857-4861 [Abstract] [Full Text]  
  • Eschenbrenner, M., Wagner, M. A., Horn, T. A., Kraycer, J. A., Mujer, C. V., Hagius, S., Elzer, P., DelVecchio, V. G. (2002). Comparative Proteome Analysis of Brucella melitensis Vaccine Strain Rev 1 and a Virulent Strain, 16M. J. Bacteriol. 184: 4962-4970 [Abstract] [Full Text]  
  • Al-Mariri, A., Tibor, A., Lestrate, P., Mertens, P., De Bolle, X., Letesson, J.-J. (2002). Yersinia enterocolitica as a Vehicle for a Naked DNA Vaccine Encoding Brucella abortus Bacterioferritin or P39 Antigen. Infect. Immun. 70: 1915-1923 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»