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Infection and Immunity, October 2001, p. 6537-6540, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6537-6540.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cloning, Nucleotide Sequence, and Expression of the Brucella melitensis sucB Gene Coding for an Immunogenic Dihydrolipoamide Succinyltransferase Homologous Protein

Michel S. Zygmunt,* María A. Díaz,dagger Ana P. Teixeira-Gomes, and Axel CloeckaertDagger

Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France

Received 12 February 2001/Returned for modification 16 May 2001/Accepted 18 July 2001

The Brucella melitensis sucB gene encoding the dihydrolipoamide succinyltransferase (E2o) enzyme (previously identified as an immunogenic protein in infected sheep) was cloned and sequenced. The amino acid sequence predicted from the cloned gene revealed 88.8 and 51.2% identity to the dihydrolipoamide succinyltransferase SucB protein from Brucella abortus and Escherichia coli, respectively. Sera from naturally infected sheep showed antibody reactivity against the recombinant SucB protein.

* Corresponding author. Mailing address: Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France. Phone: 33 2 47 42 78 72. Fax: 33 2 47 42 77 79. E-mail: zygmunt{at}tours.inra.fr.

dagger Present address: Louisiana State University, Baton Rouge, LA 70803.

Dagger Present address: Station de Pathologie Aviaire et Parasitologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France.

Infection and Immunity, October 2001, p. 6537-6540, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6537-6540.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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