This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, L.
Right arrow Articles by Reeves, P. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, L.
Right arrow Articles by Reeves, P. R.

 Previous Article  |  Next Article 

Infection and Immunity, November 2001, p. 6923-6930, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6923-6930.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Sequence Analysis of Four Shigella boydii O-Antigen Loci: Implication for Escherichia coli and Shigella Relationships

Lei Wang, Wenjia Qu, and Peter R. Reeves*

Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia

Received 11 May 2001/Returned for modification 17 July 2001/Accepted 20 August 2001

Shigella strains are in reality clones of Escherichia coli and are believed to have emerged relatively recently (G. M. Pupo, R. Lan, and P. R. Reeves, Proc. Natl. Acad. Sci. USA 97:10567-10572, 2000). There are 33 O-antigen forms in these Shigella clones, of which 12 are identical to O antigens of other E. coli strains. We sequenced O-antigen gene clusters from Shigella boydii serotypes 4, 5, 6, and 9 and also studied the O53- and O79-antigen gene clusters of E. coli, encoding O antigens identical to those of S. boydii serotype 4 and S. boydii serotype 5, respectively. In both cases the S. boydii and E. coli O-antigen gene clusters have the same genes and organization. The clusters of both S. boydii 6 and S. boydii 9 O antigens have atypical features, with a functional insertion sequence and a wzx gene located in the orientation opposite to that of all other genes in S. boydii serotype 9 and an rmlC gene located away from other rml genes in S. boydii serotype 6. Sequences of O-antigen gene clusters from another three Shigella clones have been published, and two of them also have abnormal structures, with either the entire cluster or one gene being located on a plasmid in Shigella sonnei or Shigella dysenteriae, respectively. It appears that a high proportion of clusters coding for O antigens specific to Shigella clones have atypical features, perhaps indicating recent formation of these gene clusters.

* Corresponding author. Mailing address: Department of Microbiology (GO8), The University of Sydney, Sydney, New South Wales 2006, Australia. Phone: (612) 9351 2536. Fax: (612) 9351 4571. E-mail: reeves{at}angis.org.au.

Infection and Immunity, November 2001, p. 6923-6930, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6923-6930.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wang, W., Perepelov, A. V., Feng, L., Shevelev, S. D., Wang, Q., Senchenkova, S. N., Han, W., Li, Y., Shashkov, A. S., Knirel, Y. A., Reeves, P. R., Wang, L. (2007). A group of Escherichia coli and Salmonella enterica O antigens sharing a common backbone structure. Microbiology 153: 2159-2167 [Abstract] [Full Text]  
  • Feng, L., Perepelov, A. V., Zhao, G., Shevelev, S. D., Wang, Q., Senchenkova, S. N., Shashkov, A. S., Geng, Y., Reeves, P. R., Knirel, Y. A., Wang, L. (2007). Structural and genetic evidence that the Escherichia coli O148 O antigen is the precursor of the Shigella dysenteriae type 1 O antigen and identification of a glucosyltransferase gene. Microbiology 153: 139-147 [Abstract] [Full Text]  
  • Li, Y., Liu, D., Cao, B., Han, W., Liu, Y., Liu, F., Guo, X., Bastin, D. A., Feng, L., Wang, L. (2006). Development of a Serotype-Specific DNA Microarray for Identification of Some Shigella and Pathogenic Escherichia coli Strains. J. Clin. Microbiol. 44: 4376-4383 [Abstract] [Full Text]  
  • Beutin, L., Kong, Q., Feng, L., Wang, Q., Krause, G., Leomil, L., Jin, Q., Wang, L. (2005). Development of PCR Assays Targeting the Genes Involved in Synthesis and Assembly of the New Escherichia coli O174 and O177 O Antigens. J. Clin. Microbiol. 43: 5143-5149 [Abstract] [Full Text]  
  • Li, G., Laturnus, C., Ewers, C., Wieler, L. H. (2005). Identification of Genes Required for Avian Escherichia coli Septicemia by Signature-Tagged Mutagenesis. Infect. Immun. 73: 2818-2827 [Abstract] [Full Text]  
  • Senchenkova, S. N., Feng, L., Yang, J., Shashkov, A. S., Cheng, J., Liu, D., Knirel, Y. A., Reeves, P. R., Jin, Q., Ye, Q., Wang, L. (2005). Structural and Genetic Characterization of the Shigella boydii Type 10 and Type 6 O Antigens. J. Bacteriol. 187: 2551-2554 [Abstract] [Full Text]  
  • Klena, J., Zhang, P., Schwartz, O., Hull, S., Chen, T. (2005). The Core Lipopolysaccharide of Escherichia coli Is a Ligand for the Dendritic-Cell-Specific Intercellular Adhesion Molecule Nonintegrin CD209 Receptor. J. Bacteriol. 187: 1710-1715 [Abstract] [Full Text]  
  • Lan, R., Alles, M. C., Donohoe, K., Martinez, M. B., Reeves, P. R. (2004). Molecular Evolutionary Relationships of Enteroinvasive Escherichia coli and Shigella spp.. Infect. Immun. 72: 5080-5088 [Abstract] [Full Text]  
  • Feng, L., Senchenkova, S.'y. N., Yang, J., Shashkov, A. S., Tao, J., Guo, H., Zhao, G., Knirel, Y. A., Reeves, P., Wang, L. (2004). Structural and Genetic Characterization of the Shigella boydii Type 13 O Antigen. J. Bacteriol. 186: 383-392 [Abstract] [Full Text]  
  • Russo, T. A., Davidson, B. A., Topolnycky, D. M., Olson, R., Morrill, S. A., Knight, P. R. III, Murphy, P. M. (2003). Human Neutrophil Chemotaxis Is Modulated by Capsule and O Antigen from an Extraintestinal Pathogenic Escherichia coli Strain. Infect. Immun. 71: 6435-6445 [Abstract] [Full Text]  
  • Lawrence, M. L., Banes, M. M., Azadi, P., Reeks, B. Y. (2003). The Edwardsiella ictaluri O polysaccharide biosynthesis gene cluster and the role of O polysaccharide in resistance to normal catfish serum and catfish neutrophils. Microbiology 149: 1409-1421 [Abstract] [Full Text]  
  • Wei, J., Goldberg, M. B., Burland, V., Venkatesan, M. M., Deng, W., Fournier, G., Mayhew, G. F., Plunkett, G. III, Rose, D. J., Darling, A., Mau, B., Perna, N. T., Payne, S. M., Runyen-Janecky, L. J., Zhou, S., Schwartz, D. C., Blattner, F. R. (2003). Complete Genome Sequence and Comparative Genomics of Shigella flexneri Serotype 2a Strain 2457T. Infect. Immun. 71: 2775-2786 [Abstract] [Full Text]  
  • Fukushima, M., Kakinuma, K., Kawaguchi, R. (2002). Phylogenetic Analysis of Salmonella, Shigella, and Escherichia coli Strains on the Basis of the gyrB Gene Sequence. J. Clin. Microbiol. 40: 2779-2785 [Abstract] [Full Text]  
  • Pacinelli, E., Wang, L., Reeves, P. R. (2002). Relationship of Yersinia pseudotuberculosis O Antigens IA, IIA, and IVB: the IIA Gene Cluster Was Derived from That of IVB. Infect. Immun. 70: 3271-3276 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»