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Infection and Immunity, November 2001, p. 7146-7151, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.7146-7151.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Assessment of Internalization and Viability of Porphyromonas gingivalis in KB Epithelial Cells by Confocal Microscopy

S. Houalet-Jeanne, P. Pellen-Mussi, S. Tricot-Doleux, J. Apiou, and M. Bonnaure-Mallet*

Equipe de Biologie Buccale, UPRES-EA 1256, 35000 Rennes, France

Received 16 January 2001/Returned for modification 9 May 2001/Accepted 19 June 2001

Porphyromonas gingivalis (P. gingivalis) is considered to be one of the main periodontal pathogens. The goal of this work was to confirm the ability of P. gingivalis to invade host cells. We detected P. gingivalis inside KB cells by confocal microscopy and analyzed the various aspects of the adherence and internalization process. Lysates of P. gingivalis-infected KB cells were also examined using anaerobic growth techniques. The results showed the viability and ability to replicate, inside the host cells, of the internalized pathogen. The production of vesicles was also tracked for the first time. Confocal microscopy revealed P. gingivalis in a perinuclear position.

* Corresponding author. Mailing address: Equipe de Biologie Buccale, Université de Rennes I, 2 place Pasteur, 35000 Rennes, France. Phone: 33 2 23 20 41 54. Fax: 33 2 23 20 41 09. E-mail: martine.bonnaure{at}univ-rennes1.fr.

Infection and Immunity, November 2001, p. 7146-7151, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.7146-7151.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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