This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services E-mail this article to a friend Similar articles in this journal Similar articles in ASM journals Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Celik, I. Articles by Schwaeble, W. Search for Related Content PubMed PubMed Citation Articles by Celik, I. Articles by Schwaeble, W.

 Previous Article  |  Next Article 

Infection and Immunity, December 2001, p. 7304-7309, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7304-7309.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Role of the Classical Pathway of Complement Activation in Experimentally Induced Polymicrobial Peritonitis

Ilhan Celik,¹ Cordula Stover,^2, Marina Botto,³ Steffen Thiel,⁴ Sotiria Tzima,² Dieter Künkel,¹ Mark Walport,³ Wilfried Lorenz,¹ and Wilhelm Schwaeble^2,5,*

Institute of Theoretical Surgery¹ and Department of Anatomy and Cell Biology,⁵ Philipps University Marburg, Marburg, Germany; Department of Microbiology and Immunology, University of Leicester, Leicester,² and Rheumatology Section, Hammersmith Campus, Imperial College School of Medicine, London,³ United Kingdom; and Department of Medical Microbiology and Immunology, University of Århus, Århus, Denmark⁴

Received 28 June 2001/Returned for modification 1 August 2001/Accepted 7 September 2001

The complement system and the natural antibody repertoire provide a critical first-line defense against infection. The binding of natural antibodies to microbial surfaces opsonizes invading microorganisms and activates complement via the classical pathway. Both defense systems cooperate within the innate immune response. We studied the role of the complement system in the host defense against experimental polymicrobial peritonitis using mice lacking either C1q or factor B and C2. The C1q-deficient mice lacked the classical pathway of complement activation. The factor B- and C2-deficient mice were known to lack the classical and alternative pathways, and we demonstrate here that these mice also lacked the lectin pathway of complement activation. Using inoculum doses adjusted to cause 42% mortality in the wild-type strain, none of the mice deficient in the three activation routes of complement (factor B and C2 deficient) survived (mortality of 100%). Mortality in mice deficient only in the classical pathway of complement activation (C1q deficient) was 83%. Application of further dilutions of the polymicrobial inoculum showed a dose-dependent decrease of mortality in wild-type controls, whereas no changes in mortality were observed in the two gene-targeted strains. These results demonstrate that the classical activation pathway is required for an effective antimicrobial immune defense in polymicrobial peritonitis and that, in the infection model used, the remaining antibody-independent complement activation routes (alternative and lectin pathways) provide a supporting line of defense to gain residual protection in classical pathway deficiency.

---

^* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Leicester, University Rd., Leicester LE1 9HN, United Kingdom. Phone: 0044-116-252-5674. Fax: 0044-116-252-5030. E-mail: ws5{at}le.ac.uk.

Present address: Department of Anthropology and Human Genetics, Ludwig-Maximilian-University, Munich, Germany.

---

Infection and Immunity, December 2001, p. 7304-7309, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7304-7309.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• Benhnia, M. R.-E.-I., McCausland, M. M., Laudenslager, J., Granger, S. W., Rickert, S., Koriazova, L., Tahara, T., Kubo, R. T., Kato, S., Crotty, S. (2009). Heavily Isotype-Dependent Protective Activities of Human Antibodies against Vaccinia Virus Extracellular Virion Antigen B5. J. Virol. 83: 12355-12367 [Abstract] [Full Text]  
• Mizuno, M., Ito, Y., Hepburn, N., Mizuno, T., Noda, Y., Yuzawa, Y., Harris, C. L., Morgan, B. P., Matsuo, S. (2009). Zymosan, but Not Lipopolysaccharide, Triggers Severe and Progressive Peritoneal Injury Accompanied by Complement Activation in a Rat Peritonitis Model. J. Immunol. 183: 1403-1412 [Abstract] [Full Text]  
• Yuste, J., Sen, A., Truedsson, L., Jonsson, G., Tay, L.-S., Hyams, C., Baxendale, H. E., Goldblatt, F., Botto, M., Brown, J. S. (2008). Impaired Opsonization with C3b and Phagocytosis of Streptococcus pneumoniae in Sera from Subjects with Defects in the Classical Complement Pathway. Infect. Immun. 76: 3761-3770 [Abstract] [Full Text]  
• Windbichler, M., Echtenacher, B., Hehlgans, T., Jensenius, J. C., Schwaeble, W., Mannel, D. N. (2004). Involvement of the Lectin Pathway of Complement Activation in Antimicrobial Immune Defense during Experimental Septic Peritonitis. Infect. Immun. 72: 5247-5252 [Abstract] [Full Text]  
• Carlsson, F., Berggard, K., Stalhammar-Carlemalm, M., Lindahl, G. (2003). Evasion of Phagocytosis through Cooperation between Two Ligand-binding Regions in Streptococcus pyogenes M Protein. JEM 198: 1057-1068 [Abstract] [Full Text]  
• Stahl, G. L., Xu, Y., Hao, L., Miller, M., Buras, J. A., Fung, M., Zhao, H. (2003). Role for the Alternative Complement Pathway in Ischemia/Reperfusion Injury. Am. J. Pathol. 162: 449-455 [Abstract] [Full Text]  
• Brown, J. S., Hussell, T., Gilliland, S. M., Holden, D. W., Paton, J. C., Ehrenstein, M. R., Walport, M. J., Botto, M. (2002). The classical pathway is the dominant complement pathway required for innate immunity to Streptococcus pneumoniae infection in mice. Proc. Natl. Acad. Sci. USA 99: 16969-16974 [Abstract] [Full Text]