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Infection and Immunity, December 2001, p. 7603-7609, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7603-7609.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

High Prevalence of Human Antibodies to Recombinant Duffy Binding-Like alpha  Domains of the Plasmodium falciparum-Infected Erythrocyte Membrane Protein 1 in Semi-Immune Adults Compared to That in Nonimmune Children

Raphael M. Oguariri,1 Steffen Borrmann,1,2 Mo-Quen Klinkert,1 Peter G. Kremsner,1,2 and Jürgen F. J. Kun1,*

Department of Parasitology, Institute for Tropical Medicine, University of Tübingen, 72074 Tübingen, Germany,1 and Medical Research Unit, Albert-Schweitzer Hospital, Lambaréné, Gabon2

Received 10 May 2001/Returned for modification 7 July 2001/Accepted 25 August 2001

We used a panel of nine fusion proteins that contain different Duffy binding-like alpha  (DBL-alpha ) domains of Plasmodium falciparum-infected erythrocyte membrane protein 1 to assess the levels of antibody activity in serum samples obtained from semi-immune or nonimmune individuals from Lambaréné, Gabon. Recognition was measured in terms of either the prevalence or the magnitude of the response. A strong correlation between the immune status of the patients and reactivity with recombinant proteins was observed, which was interpreted as a reflection of the number of infections acquired over time. The antibody responses were predominantly directed toward variable epitopes of the DBL-alpha domain. Antibody responses could be reduced by preincubation of the sera with various fusion proteins. A portion of individuals who exhibited high-level responses to all fusion proteins also had antibodies which recognized conserved epitopes. The possibility that a synergizing effect of anti-DBL-alpha domain antibodies could support chemotherapy is discussed.

* Corresponding author. Mailing address: Department of Parasitology, Institute for Tropical Medicine, University of Tübingen, Wilhelmstrasse 27, 72074 Tübingen, Germany. Phone: 49-7071-2980240. Fax: 49-7071-295189. E-mail: juergen.kun{at}uni-tuebingen.de.

Infection and Immunity, December 2001, p. 7603-7609, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7603-7609.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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