This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Triglia, T.
Right arrow Articles by Cowman, A. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Triglia, T.
Right arrow Articles by Cowman, A. F.

 Previous Article  |  Next Article 

Infection and Immunity, February 2001, p. 1084-1092, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.1084-1092.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Proteins from Plasmodium falciparum That Are Homologous to Reticulocyte Binding Proteins in Plasmodium vivax

Tony Triglia, Jenny Thompson, Sonia R. Caruana, Mauro Delorenzi, Terry Speed, and Alan F. Cowman*

The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Victoria, Australia

Received 31 July 2000/Returned for modification 9 October 2000/Accepted 24 October 2000

Plasmodium falciparum infections can be fatal, while P. vivax infections usually are not. A possible factor involved in the greater virulence of P. falciparum is that this parasite grows in red blood cells (RBCs) of all maturities whereas P. vivax is restricted to growth in reticulocytes, which represent only approximately 1% of total RBCs in the periphery. Two proteins, expressed at the apical end of the invasive merozoite stage from P. vivax, have been implicated in the targeting of reticulocytes for invasion by this parasite. A search of the P. falciparum genome databases has identified genes that are homologous to the P. vivax rbp-1 and -2 genes. Two of these genes are virtually identical over a large region of the 5' end but are highly divergent at the 3' end. They encode high-molecular-mass proteins of >300 kDa that are expressed in late schizonts and localized to the apical end of the merozoite. To test a potential role in merozoite invasion of RBCs, we analyzed the ability of these proteins to bind to mature RBCs and reticulocytes. No binding to mature RBCs or cell preparations enriched for reticulocytes was detected. We identified a parasite clone that lacks the gene for one of these proteins, showing that the gene is not required for normal in vitro growth. Antibodies to these proteins can inhibit merozoite invasion of RBCs.

* Corresponding author. Mailing address: The Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital, Victoria 3050, Australia. Phone: 61-3-9345 2555. Fax: 61-3-9347 0852. E-mail: cowman{at}wehi.edu.au.

Infection and Immunity, February 2001, p. 1084-1092, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.1084-1092.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tham, W.-H., Wilson, D. W., Reiling, L., Chen, L., Beeson, J. G., Cowman, A. F. (2009). Antibodies to Reticulocyte Binding Protein-Like Homologue 4 Inhibit Invasion of Plasmodium falciparum into Human Erythrocytes. Infect. Immun. 77: 2427-2435 [Abstract] [Full Text]  
  • Haase, S., Cabrera, A., Langer, C., Treeck, M., Struck, N., Herrmann, S., Jansen, P. W., Bruchhaus, I., Bachmann, A., Dias, S., Cowman, A. F., Stunnenberg, H. G., Spielmann, T., Gilberger, T.-W. (2008). Characterization of a Conserved Rhoptry-Associated Leucine Zipper-Like Protein in the Malaria Parasite Plasmodium falciparum. Infect. Immun. 76: 879-887 [Abstract] [Full Text]  
  • Jennings, C. V., Ahouidi, A. D., Zilversmit, M., Bei, A. K., Rayner, J., Sarr, O., Ndir, O., Wirth, D. F., Mboup, S., Duraisingh, M. T. (2007). Molecular Analysis of Erythrocyte Invasion in Plasmodium falciparum Isolates from Senegal. Infect. Immun. 75: 3531-3538 [Abstract] [Full Text]  
  • Narum, D. L., Ogun, S. A., Batchelor, A. H., Holder, A. A. (2006). Passive Immunization with a Multicomponent Vaccine against Conserved Domains of Apical Membrane Antigen 1 and 235-Kilodalton Rhoptry Proteins Protects Mice against Plasmodium yoelii Blood-Stage Challenge Infection.. Infect. Immun. 74: 5529-5536 [Abstract] [Full Text]  
  • TRAN, T. M., OLIVEIRA-FERREIRA, J., MORENO, A., SANTOS, F., YAZDANI, S. S., CHITNIS, C. E., ALTMAN, J. D., MEYER, E. V-S., BARNWELL, J. W., GALINSKI, M. R. (2005). COMPARISON OF IgG REACTIVITIES TO PLASMODIUM VIVAX MEROZOITE INVASION ANTIGENS IN A BRAZILIAN AMAZON POPULATION. Am J Trop Med Hyg 73: 244-255 [Abstract] [Full Text]  
  • RAYNER, J. C., TRAN, T. M., CORREDOR, V., HUBER, C. S., BARNWELL, J. W., GALINSKI, M. R. (2005). DRAMATIC DIFFERENCE IN DIVERSITY BETWEEN PLASMODIUM FALCIPARUM AND PLASMODIUM VIVAX RETICULOCYTE BINDING-LIKE GENES. Am J Trop Med Hyg 72: 666-674 [Abstract] [Full Text]  
  • Cowman, A. F., Crabb, B. S. (2002). The Plasmodium falciparum Genome-- a Blueprint for Erythrocyte Invasion. Science 298: 126-128 [Abstract] [Full Text]  
  • Taylor, H. M., Grainger, M., Holder, A. A. (2002). Variation in the Expression of a Plasmodium falciparum Protein Family Implicated in Erythrocyte Invasion. Infect. Immun. 70: 5779-5789 [Abstract] [Full Text]  
  • Rayner, J. C., Vargas-Serrato, E., Huber, C. S., Galinski, M. R., Barnwell, J. W. (2001). A Plasmodium falciparum Homologue of Plasmodium vivax Reticulocyte Binding Protein (PvRBP1) Defines a Trypsin-resistant Erythrocyte Invasion Pathway. JEM 194: 1571-1582 [Abstract] [Full Text]  
  • Taylor, H. M., Triglia, T., Thompson, J., Sajid, M., Fowler, R., Wickham, M. E., Cowman, A. F., Holder, A. A. (2001). Plasmodium falciparum Homologue of the Genes for Plasmodium vivax and Plasmodium yoelii Adhesive Proteins, Which Is Transcribed but Not Translated. Infect. Immun. 69: 3635-3645 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»