This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neary, J. M.
Right arrow Articles by Murphy, T. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neary, J. M.
Right arrow Articles by Murphy, T. F.

 Previous Article  |  Next Article 

Infection and Immunity, February 2001, p. 773-778, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.773-778.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Antibodies to Loop 6 of the P2 Porin Protein of Nontypeable Haemophilus influenzae Are Bactericidal against Multiple Strains

John M. Neary,1 Kyungcheol Yi,1 Richard J. Karalus,1 and Timothy F. Murphy1,2,3,*

Department of Microbiology1 and Division of Infectious Diseases, Department of Medicine,2 State University of New York at Buffalo, and Veterans Affairs Western New York Healthcare System,3 Buffalo, New York 14215

Received 22 May 2000/Returned for modification 5 July 2000/Accepted 11 November 2000

The P2 porin protein is the most abundant protein in the outer membrane of nontypeable Haemophilus influenzae (NTHI). Analysis of sequences of P2 from different strains reveals the presence of both heterogeneous and conserved surface-exposed loops of the P2 molecule among strains. The present study was undertaken to test the hypothesis that antibodies to a conserved surface-exposed loop are bactericidal for multiple strains of NTHI and could thus form the basis of vaccines to prevent infection due to NTHI. Polyclonal antiserum to a peptide corresponding to loop 6 was raised and was immunopurified over a loop 6 peptide column. Analysis of the antibodies to whole organisms and peptides corresponding to each of the eight loops of P2 by immunoassays revealed that the antibodies were highly specific for loop 6 of P2. The immunopurified antibodies bound to P2 of 14 of 15 strains in immunoblot assays. These antibodies to loop 6 demonstrated complement-mediated bactericidal killing of 8 of 15 strains. These results support the concept of using conserved regions of the P2 protein as a vaccine antigen.

* Corresponding author. Mailing address: VA Western New York Healthcare System, Medical Research 151, 3495 Bailey Ave., Buffalo, NY 14215. Phone: (716) 862-7874. Fax: (716) 862-6526. E-mail: murphyt{at}acsu.buffalo.edu.

Infection and Immunity, February 2001, p. 773-778, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.773-778.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pang, B., Winn, D., Johnson, R., Hong, W., West-Barnette, S., Kock, N., Swords, W. E. (2008). Lipooligosaccharides Containing Phosphorylcholine Delay Pulmonary Clearance of Nontypeable Haemophilus influenzae. Infect. Immun. 76: 2037-2043 [Abstract] [Full Text]  
  • Prather, D. T., Bains, M., Hancock, R. E. W., Filiatrault, M. J., Campagnari, A. A. (2004). Differential Expression of Porins OmpP2A and OmpP2B of Haemophilus ducreyi. Infect. Immun. 72: 6271-6278 [Abstract] [Full Text]  
  • Novotny, L. A., Bakaletz, L. O. (2003). The Fourth Surface-Exposed Region of the Outer Membrane Protein P5-Homologous Adhesin of Nontypable Haemophilus influenzae Is an Immunodominant But Nonprotective Decoying Epitope. J. Immunol. 171: 1978-1983 [Abstract] [Full Text]  
  • Galdiero, S., Capasso, D., Vitiello, M., D'Isanto, M., Pedone, C., Galdiero, M. (2003). Role of Surface-Exposed Loops of Haemophilus influenzae Protein P2 in the Mitogen-Activated Protein Kinase Cascade. Infect. Immun. 71: 2798-2809 [Abstract] [Full Text]  
  • Davies, R. L., MacCorquodale, R., Baillie, S., Caffrey, B. (2003). Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis. J Med Microbiol 52: 59-67 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»