This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jansen, W. T. M.
Right arrow Articles by Verheul, A. F. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jansen, W. T. M.
Right arrow Articles by Verheul, A. F. M.

 Previous Article  |  Next Article 

Infection and Immunity, February 2001, p. 787-793, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.787-793.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Synthetic 6B Di-, Tri-, and Tetrasaccharide-Protein Conjugates Contain Pneumococcal Type 6A and 6B Common and 6B- Specific Epitopes That Elicit Protective Antibodies in Mice

Wouter T. M. Jansen,1,* Sietske Hogenboom,1 Mark J. L. Thijssen,2 Johannis P. Kamerling,2 Johannes F. G. Vliegenthart,2 Jan Verhoef,1 Harm Snippe,1 and André F. M. Verheul1,dagger

Vaccines Section, Eijkman-Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, Utrecht University Hospital, 3584 CX Utrecht,1 and Department of Bio-Organic Chemistry, Bijvoet Center, Utrecht University, 3584 CH Utrecht,2 The Netherlands

Received 8 August 2000/Returned for modification 6 September 2000/Accepted 31 October 2000

The immunogenicity and protective capacity of Streptococcus pneumoniae 6B capsular polysaccharide (PS)-derived synthetic phosphate-containing disaccharide (Rha-ribitol-P-), trisaccharide (ribitol-P-Gal-Glc-), and tetrasaccharide (Rha-ribitol-P-Gal-Glc-)-protein conjugates in rabbits and mice were studied. In rabbits, all saccharides conjugated to keyhole limpet hemocyanin (KLH) evoked high levels of pneumococcal (Pn) type 6B antibodies that facilitated type-specific phagocytosis. Unlike the disaccharide rabbit antisera, tri- and tetrasaccharide rabbit antisera also reacted with 6A PS in an enzyme-linked immunosorbent assay (ELISA) and promoted phagocytosis of 6A pneumococci. All these rabbit antisera passively protected mice against a Pn 6B challenge. The disaccharide conjugate-induced antiserum, however, failed to protect mice against a 6A challenge. In mice, phagocytic and protective anti-Pn 6B antibodies were only induced by the tetrasaccharide conjugate and not by PS 6B or PS 6B-protein conjugates. These antibodies did not cross-react with 6A PS in ELISA and were unable to phagocytize 6A pneumococci. In conclusion, the disaccharide and tetrasaccharide conjugates already contain epitopes capable of inducing 6B-specific, fully protective antibodies in rabbits and mice, respectively.

* Corresponding author. Present address: GBF, Section Microbiology D209, D-38124 Braunschweig, Germany. Phone: 0049 531 6181706. Fax: 0049 531 6181708. E-mail: JWO{at}GBF.de.

dagger Present address: Intervet International, Boxmeer, The Netherlands.

Infection and Immunity, February 2001, p. 787-793, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.787-793.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Park, S., Parameswar, A. R., Demchenko, A. V., Nahm, M. H. (2009). Identification of a Simple Chemical Structure Associated with Protective Human Antibodies against Multiple Pneumococcal Serogroups. Infect. Immun. 77: 3374-3379 [Abstract] [Full Text]  
  • Phalipon, A., Costachel, C., Grandjean, C., Thuizat, A., Guerreiro, C., Tanguy, M., Nato, F., Vulliez-Le Normand, B., Belot, F., Wright, K., Marcel-Peyre, V., Sansonetti, P. J., Mulard, L. A. (2006). Characterization of Functional Oligosaccharide Mimics of the Shigella flexneri Serotype 2a O-Antigen: Implications for the Development of a Chemically Defined Glycoconjugate Vaccine. J. Immunol. 176: 1686-1694 [Abstract] [Full Text]  
  • Geyer, H., Wuhrer, M., Resemann, A., Geyer, R. (2005). Identification and Characterization of Keyhole Limpet Hemocyanin N-Glycans Mediating Cross-reactivity with Schistosoma mansoni. J. Biol. Chem. 280: 40731-40748 [Abstract] [Full Text]  
  • Lefeber, D. J., Benaissa-Trouw, B., Vliegenthart, J. F. G., Kamerling, J. P., Jansen, W. T. M., Kraaijeveld, K., Snippe, H. (2003). Th1-Directing Adjuvants Increase the Immunogenicity of Oligosaccharide-Protein Conjugate Vaccines Related to Streptococcus pneumoniae Type 3. Infect. Immun. 71: 6915-6920 [Abstract] [Full Text]  
  • May, R. J., Beenhouwer, D. O., Scharff, M. D. (2003). Antibodies to Keyhole Limpet Hemocyanin Cross-React with an Epitope on the Polysaccharide Capsule of Cryptococcus neoformans and Other Carbohydrates: Implications for Vaccine Development. J. Immunol. 171: 4905-4912 [Abstract] [Full Text]  
  • Mawas, F., Niggemann, J., Jones, C., Corbel, M. J., Kamerling, J. P., Vliegenthart, J. F. G. (2002). Immunogenicity in a Mouse Model of a Conjugate Vaccine Made with a Synthetic Single Repeating Unit of Type 14 Pneumococcal Polysaccharide Coupled to CRM197. Infect. Immun. 70: 5107-5114 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»