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Infection and Immunity, February 2001, p. 924-930, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.924-930.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Systemic and Mucosal Immunizations with Fibronectin-Binding Protein FBP54 Induce Protective Immune Responses against Streptococcus pyogenes Challenge in Mice

Shigetada Kawabata,1,* Eiji Kunitomo,1 Yutaka Terao,1 Ichiro Nakagawa,1 Ken Kikuchi,2 Kyo-ichi Totsuka,2 and Shigeyuki Hamada1

Department of Oral Microbiology, Osaka University Faculty of Dentistry, Suita-Osaka 565-0871,1 and Department of Infectious Diseases, Tokyo Women's Medical University Shinjuku-ku, Tokyo 162-8666,2 Japan

Received 3 August 2000/Returned for modification 18 October 2000/Accepted 7 November 2000

The purpose of this study was to examine the suitability of fibronectin-binding protein FBP54 as a putative vaccine for Streptococcus pyogenes infections. When the distribution of the fbp54 gene among the clinical isolates representing various M serotypes was tested by PCR and Southern blot assays, it was found that all of the strains possess this gene. Furthermore, a significant increase in immunoglobulin G (IgG) antibody titers against FBP54 was observed in sera from patients with S. pyogenes infections compared with those from healthy volunteers (P < 0.005). Mice were immunized with FBP54 subcutaneously, orally, or nasally. An enzyme-linked immunosorbent assay revealed that antigen-specific IgG antibodies were induced in the sera of immunized mice, while high salivary levels of IgA antibodies were detected after oral and nasal immunizations. Mice subcutaneously or orally immunized with FBP54 survived significantly longer following the challenge with S. pyogenes than did nonimmunized mice (P < 0.001). These results indicate that FBP54 is a promising vaccine for the prevention of S. pyogenes infections.

* Corresponding author. Mailing address: Department of Oral Microbiology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita-Osaka 565-0871, Japan. Phone: 81-6-6879-2898. Fax: 81-6-6878-4755. E-mail: kawabata{at}dent.osaka-u.ac.jp.

Infection and Immunity, February 2001, p. 924-930, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.924-930.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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