Previous Article | Next Article 
Infection and Immunity, March 2001, p. 1599-1604, Vol. 69, No. 3
Departments of
Parasitology,1
Biochemistry,4 and
Microbiology5 and Medical
Research Center,2 College of Medicine, Ewha
Woman's University, Seoul 158-710, Korea, and Department of
Immunology and Internal Medicine, Mayo Clinic and Mayo Foundation,
Rochester, Minnesota 559053
Received 27 September 2000/Returned for modification 1 December
2000/Accepted 15 December 2000
An immunoglobulin G (IgG)-coated surface, such as that found on
helminth parasites, is one of the most effective physiologic stimuli
for eosinophil activation. The cysteine proteases secreted by
tissue-invasive helminth larvae play an important role in evasion of
the immune response by degrading the host immunoglobulins. In this
study, we investigated whether cysteine proteases in the excretory-secretory product (ESP) produced by Paragonimus
westermani newly excysted metacercariae (PwNEM), which cause
pulmonary or extrapulmonary paragonimiasis in human beings, could
modify effector functions of human eosinophils stimulated with IgG. We
coated 96-well plates with human IgG in the absence or presence of the ESP produced by PwNEM. When eosinophils were incubated in the wells
coated with IgG in the presence of the ESP, eosinophil degranulation and superoxide production were significantly reduced compared with
results for cells incubated in wells coated with IgG alone. This
inhibitory effect of the ESP on IgG-induced superoxide production was
dose dependent and was significantly abolished by pretreatment of the
ESP with heat. These findings suggest that the cysteine proteases
secreted by PwNEM attenuate both activation and degranulation of
eosinophils stimulated with IgG. Thus, the cysteine proteases produced
by tissue-invasive helminth larvae play crucial roles in evasion of
IgG-dependent eosinophil helminthotoxicity and in reduction of
eosinophil-associated tissue inflammation during the migratory period.
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.3.1599-1604.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Cysteine Protease Secreted by Paragonimus
westermani Attenuates Effector Functions of Human Eosinophils
Stimulated with Immunoglobulin G
*
Corresponding author. Mailing address: Department of
Parasitology, College of Medicine, Ewha Woman's University, 911-1, Mok-6-Dong, Yangcheon-Gu, Seoul 158-710, Korea. Phone: 82-2-650-5750. Fax: 82-2-653-8891. E-mail: mhshin{at}mm.ewha.ac.kr.
Infection and Immunity, March 2001, p. 1599-1604, Vol. 69, No. 3
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.3.1599-1604.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Procop, G. W.
(2009). North American Paragonimiasis (Caused by Paragonimus kellicotti) in the Context of Global Paragonimiasis. Clin. Microbiol. Rev.
22: 415-446
[Abstract] [Full Text] -
Collin, M., Olsen, A.
(2003). Extracellular Enzymes with Immunomodulating Activities: Variations on a Theme in Streptococcus pyogenes. Infect. Immun.
71: 2983-2992
[Full Text] -
Collin, M., Svensson, M. D., Sjoholm, A. G., Jensenius, J. C., Sjobring, U., Olsen, A.
(2002). EndoS and SpeB from Streptococcus pyogenes Inhibit Immunoglobulin-Mediated Opsonophagocytosis. Infect. Immun.
70: 6646-6651
[Abstract] [Full Text] -
Collin, M., Olsen, A.
(2001). Effect of SpeB and EndoS from Streptococcus pyogenes on Human Immunoglobulins. Infect. Immun.
69: 7187-7189
[Abstract] [Full Text]
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»