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Infection and Immunity, March 2001, p. 1902-1908, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1902-1908.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Composition and Gene Expression of the cag Pathogenicity Island in Helicobacter pylori Strains Isolated from Gastric Carcinoma and Gastritis Patients in Costa Rica

Alessandra Occhialini,1 Armelle Marais,1 Maria Urdaci,2 Rafaela Sierra,3 Nubia Muñoz,4 Antonello Covacci,5 and Francis Mégraud1,*

Laboratoire de Bactériologie, Université Victor Ségalen Bordeaux 2 et Hôpital Pellegrin, 33076 Bordeaux,1 Laboratoire de Microbiologie, Ecole Nationale d'Ingénieurs des Travaux Agricoles, 33170 Gradignan,2 and Centre International de Recherche sur le Cancer, 69372 Lyon,4 France; University of Costa Rica, San José, Costa Rica;3 and IRIS, 53100 Siena, Italy5

Received 7 August 2000/Returned for modification 25 September 2000/Accepted 20 November 2000

The composition and in vitro expression of the cag pathogenicity island genes in a group of Helicobacter pylori strains obtained from patients suffering from chronic gastritis-associated dyspepsia (n = 26) or gastric carcinoma (n = 17) were analyzed. No significant difference in the distribution of the 10 studied regions was found between the cases and the controls. Nine strains did not harbor any of the selected regions: eight (30.8%) isolated from patients with gastritis only and one (5.9%) from a patient with gastric carcinoma. No association was found between the number of repeated sequences at the 3' end of the cagA gene or the presence of tyrosine phosphorylation motifs and the clinical origin of the strains. The virB10 homolog gene was the sole gene studied to be significantly expressed more often in cancer strains than in gastritis strains (P = 0.03).

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Hôpital Pellegrin, Place Amélie Raba-Léon, 33076 Bordeaux Cedex, France. Phone: 33-5-56-79-59-10. Fax: 33-5-56-79-60-18. E-mail: francis.megraud{at}chubordeaux.fr.

Infection and Immunity, March 2001, p. 1902-1908, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1902-1908.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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