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Infection and Immunity, March 2001, p. 1943-1946, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1943-1946.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Acapsular Pasteurella multocida B:2 Can Stimulate Protective Immunity against Pasteurellosis

John D. Boyce and Ben Adler*

Bacterial Pathogenesis Research Group, Department of Microbiology, School of Biomedical Sciences, Monash University, Victoria, 3800, Australia

Received 20 September 2000/Returned for modification 24 October 2000/Accepted 20 November 2000

We have previously shown that a Pasteurella multocida cexA mutant (PBA875) was impaired in capsule export and highly attenuated in virulence for mice (J. D. Boyce and B. Adler, Infect. Immun. 68:3463-3468, 2000). In this study we show that immunization with high, but not low, doses of PBA875 can confer significant protection against wild-type challenge. We have also constructed a genetically defined acapsular P. multocida strain (AL18) by inactivation of bcbH, a gene predicted to be involved in polysaccharide biosynthesis. AL18 failed to produce immunoreactive polysaccharide as determined by immunofluorescence and Western immunoblot. Immunization of mice with live AL18 conferred significant protection against wild-type challenge, while immunization with similar doses of either killed wild-type or killed AL18 failed to confer protection.

* Corresponding author. Mailing address: Bacterial Pathogenesis Research Group, Department of Microbiology, School of Biomedical Sciences, Monash University, Victoria, 3800, Australia. Phone: 61-3-9905-4815. Fax: 61-3-9905-4811. E-mail: ben.adler{at}med.monash.edu.au.

Infection and Immunity, March 2001, p. 1943-1946, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1943-1946.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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