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Infection and Immunity, April 2001, p. 2031-2036, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2031-2036.2001

Mucosal Vaccination with Recombinantly Attenuated Staphylococcal Enterotoxin B and Protection in a Murine Model

Bradley G. Stiles,* Anthony R. Garza, Robert G. Ulrich, and James W. Boles

Toxinology and Aerobiology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702-5011

Received 25 September 2000/Returned for modification 14 December 2000/Accepted 3 January 2001

Previous work in our laboratory revealed that mice parenterally vaccinated with recombinantly attenuated staphylococcal enterotoxin (SE) or toxic shock syndrome toxin 1 develop protective antibodies against a lethal intraperitoneal (i.p.) toxin challenge. This study investigated the efficacy of nasal and oral immunizations with an SEB vaccine (SEBv) toward an i.p. or mucosal (via an aerosol) toxin challenge. Both vaccination routes, with the immunoadjuvant cholera toxin (CT), elicited comparable SEB-specific immunoglobulin A (IgA) and IgG levels in saliva. Nasal or oral inoculations also generated SEB-specific IgA, IgG, and IgM in the serum, but the nasal route yielded higher specific IgG titers. SEBv alone, when given nasally or orally, did not induce any detectable SEB-specific antibody. Mice vaccinated mucosally were protected against a 50% lethal dose of wild-type SEB given i.p. or mucosally, thus demonstrating that nasal or oral administration of this SEBv, with CT, elicits systemic and mucosal antibodies to SEB that protect against SEB-induced lethal shock.

* Corresponding author. Mailing address: Toxinology and Aerobiology Division, Department of Immunology and Molecular Biology, USAMRIID, Fort Detrick, MD 21702-5011. Phone: (301) 619-4809. Fax: (301) 619-2348. E-mail: bradley.stiles{at}amedd.army.mil.

Infection and Immunity, April 2001, p. 2031-2036, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2031-2036.2001


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