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Infection and Immunity, April 2001, p. 2396-2401, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2396-2401.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Profiles of Immunoglobulin M (IgM) and IgG Antibodies against Defined Carbohydrate Epitopes in Sera of Schistosoma-Infected Individuals Determined by Surface Plasmon Resonance

Alexandra van Remoortere,1,2 Govert J. van Dam,1 Cornelis H. Hokke,1 Dirk H. van den Eijnden,2 Irma van Die,2 and André M. Deelder1,*

Department of Parasitology, Leiden University Medical Center, 2300 RC Leiden,1 and Department of Medical Chemistry, Vrije Universiteit, 1081 BT Amsterdam,2 The Netherlands

Received 3 November 2000/Returned for modification 15 December 2000/Accepted 19 January 2001

We report here that sera of children and adults infected with Schistosoma mansoni, S. haematobium, or S. japonicum contain antibodies against GalNAcbeta 1-4(Fucalpha 1-2Fucalpha 1-3)GlcNAc (LDN-DF) and to a lesser extent to Galbeta 1-4(Fucalpha 1-3)GlcNAc (Lewisx) and GalNAcbeta 1-4GlcNAc (LDN). Surface plasmon resonance (SPR) spectroscopy was used to monitor the presence of serum antibodies to neoglycoconjugates containing these carbohydrate epitopes and to define the immunoglobulin M (IgM) and IgG subclass distribution of the antibodies. The serum levels of antibodies to LDN-DF are high related to LDN and Lewisx for all examined groups of Schistosoma-infected individuals. A higher antibody response to the LDN-DF epitope was found in sera of infected children than in sera of infected adults regardless of the schistosome species. With respect to the subclasses, we found surprisingly that individuals infected with S. japonicum have predominantly IgG antibodies, while individuals infected with S. mansoni mainly show an IgM response; high levels of both isotypes were measured in sera of individuals infected with S. haematobium. These data provide new insights in the human humoral immune response to schistosome-derived glycans.

* Corresponding author. Mailing address: Department of Parasitology, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Phone: 31715265062. Fax: 31715266907. E-mail: a.m.deelder{at}lumc.nl.

Infection and Immunity, April 2001, p. 2396-2401, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2396-2401.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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