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Infection and Immunity, April 2001, p. 2659-2665, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2659-2665.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Curli Fibers Mediate Internalization of Escherichia coli by Eukaryotic Cells

U. Gophna,1 M. Barlev,1 R. Seijffers,1 T. A. Oelschlager,2 J. Hacker,2 and E. Z. Ron1,*

Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel,1 and Institut für Molekulare Infektionsbiologie, 97070 Würzburg, Germany2

Received 18 September 2000/Returned for modification 7 December 2000/Accepted 16 January 2001

Curli fibers are adhesive surface fibers expressed by Escherichia coli and Salmonella enterica that bind several host extracellular matrix and contact phase proteins and were assumed to have a role in pathogenesis. The results presented here suggest that one such role is internalization into host cells. An E. coli K-12 strain transformed with a low-copy vector containing the gene cluster encoding curli fibers (csg operon) was internalized by several lines of eukaryotic cells. The internalization could be correlated with a high level of curli fiber expression and was abolished by disruption of the csg operon. The ability to be internalized by eukaryotic cells could be conferred even by the curli fiber gene cluster of a noninvasive K-12 strain, but the homologous csg cluster from a virulent septicemic E. coli isolate mediated a higher level of internalization. The finding that curli fibers promote bacterial internalization indicates a new role for curli fibers in pathogenesis.

* Corresponding author. Mailing address: Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel. Phone: 972 (3) 640 9379. Fax: 972 (3) 641 4138. E-mail: eliora{at}post.tau.ac.il.

Infection and Immunity, April 2001, p. 2659-2665, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2659-2665.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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