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Infection and Immunity, May 2001, p. 3013-3020, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3013-3020.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Expression of pls, a Gene Closely Associated with the mecA Gene of Methicillin-Resistant Staphylococcus aureus, Prevents Bacterial Adhesion In Vitro

K. Savolainen,1,* L. Paulin,2 B. Westerlund-Wikström,1,dagger T. J. Foster,3 T. K. Korhonen,1 and P. Kuusela4,5

Department of Biosciences, Division of General Microbiology,1 Institute of Biotechnology,2 and Department of Bacteriology and Immunology, The Haartman Institute,4 00014 University of Helsinki, and Division of Clinical Microbiology, HUCH Laboratory Diagnostics, Helsinki University Central Hospital, Helsinki,5 Finland, and Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin, Ireland3

Received 6 October 2000/Returned for modification 11 December 2000/Accepted 2 February 2001

The pls gene, coding for a large surface protein of methicillin-resistant Staphylococcus aureus, was cloned from a strain which adheres poorly to several mammalian proteins. The structure of pls revealed three distinct repeat regions, one of which was a serine-aspartate repeat characteristic of the Clf-Sdr family of surface proteins in staphylococci. The lengths of the repeat regions varied in different clinical strains and could be used as epidemiological markers. pls was found to be closely associated with the mecA gene by pulsed-field gel electrophoresis analysis of SmaI-digested DNA. A pls mutant constructed by allele replacement adhered well to immobilized fibronectin and immunoglobulin G, in contrast to the parental strain, suggesting that Pls could have a role in preventing adhesion at some stages during an infection.

* Corresponding author. Mailing address: Department of Biosciences, Division of General Microbiology, P.O. Box 56, 00014 University of Helsinki, Finland. Phone: 358-9-191 59246. Fax: 358-9-191 59262. E-mail: Katri.M.Savolainen{at}helsinki.fi.

dagger Present address: Orion Diagnostica, Espoo, Finland.

Infection and Immunity, May 2001, p. 3013-3020, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3013-3020.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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