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Infection and Immunity, May 2001, p. 3413-3417, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3413-3417.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Immunohistochemical Analysis of Cellular Infiltrate and Gamma Interferon, Interleukin-12, and Inducible Nitric Oxide Synthase Expression in Leprosy Type 1 (Reversal) Reactions before and during Prednisolone Treatment

David Little,1 Saroj Khanolkar-Young,1 Anne Coulthart,1 Sujai Suneetha,2 and Diana N. J. Lockwood1,*

Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom,1 and Dhoolpet Leprosy Research Centre, Hyderabad, India2

Received 1 September 2000/Returned for modification 30 December 2000/Accepted 22 February 2001

The effects of prednisolone treatment on the cellularity and cytokine (gamma interferon, interleukin-12, and inducible nitric oxide synthase) profiles of leprosy skin type 1 (reversal) reactions were studied using immunohistochemistry. Skin biopsies were taken from 15 patients with leprosy type 1 (reversal) reactions at days 0, 7, 28, and 180 after the start of steroid treatment. Prednisolone treatment had little effect at day 7, but by day 28 significant decreases were found in cytokine levels. Some patients maintained cytokine production at days 28 and 180. These results illustrate the strong Th1 profile of type 1 reactional lesions, the slow response to steroid therapy, and continuing activity at 180 days.

* Corresponding author. Mailing address: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St., London, WCIE 7HT, United Kingdom. Phone: 020 7927 2457. Fax: 020 7637 4314. E-mail: Diana.Lockwood{at}lshtm.ac.uk.

Infection and Immunity, May 2001, p. 3413-3417, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3413-3417.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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