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Infection and Immunity, May 2001, p. 3502-3506, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3502-3506.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of Proteins in the Outer Membrane Preparation of a Murine Pathogen, Helicobacter bilis

Zhongming Ge,1 Peter Doig,2 and James G. Fox1,*

Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge,1 and AstraZeneca R&D Boston, Waltham,2 Massachusetts

Received 29 December 2000/Accepted 1 February 2001

Helicobacter bilis is a bacterial pathogen associated with multifocal hepatitis and inflammatory bowel disease in certain strains of mice. This bacterium colonizes the liver, bile, and lower intestine in mice and has also been isolated from a wide spectrum of laboratory animals. In this study, proteins present in the outer membrane preparation (OMP) of four H. bilis strains isolated from a mouse, a dog, a rat, and a gerbil were characterized and compared with that of Helicobacter pylori, a human gastric pathogen. All four H. bilis strains had similar OMP protein profiles that were distinct from those of H. pylori. Immunoblotting demonstrated that OMP proteins from H. bilis and H. pylori have little cross-reactivity, except for their flagellins. Nine major immunogenic polypeptides were present in the H. bilis OMPs. By using two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis, five heat-modifiable proteins with molecular masses of 82, 66, 52, 47 and 37 kDa were identified. The N-terminal sequences of the 46- and 47-kDa OMP proteins had no homology with protein sequences available in public databases. These results indicate that H. bilis has a conserved, unique OMP protein profile that is distinct from those of H. pylori.

* Corresponding author. Mailing address: Division of Comparative Medicine, Massachusetts Institute of Technology, 16-825, 77 Massachusetts Ave., Cambridge, MA 02139. Phone: (617) 253-1735. Fax: (617) 258-5708. E-mail: jgfox{at}mit.edu.

Infection and Immunity, May 2001, p. 3502-3506, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3502-3506.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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