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Infection and Immunity, July 2001, p. 4681-4685, Vol. 69, No. 7
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.7.4681-4685.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Vibrio cholerae tolC Is Required for Bile Resistance and Colonization

James E. Bina and John J. Mekalanos*

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115

Received 11 December 2000/Returned for modification 27 February 2001/Accepted 2 April 2001

TolC and its homologues are outer membrane proteins that are essential for the transport of many molecules across the cell envelope. In this study we characterized the gene encoding Vibrio cholerae TolC. V. cholerae tolC mutants failed to secrete the RTX cytotoxin, were hypersensitive to antimicrobial agents, and were deficient in intestinal colonization.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115. Phone: (617) 432-1935. Fax: (617) 738-7664. E-mail: John_Mekalanos{at}hms.harvard.edu.

Infection and Immunity, July 2001, p. 4681-4685, Vol. 69, No. 7
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.7.4681-4685.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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