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Infection and Immunity, July 2001, p. 4686-4690, Vol. 69, No. 7
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.7.4686-4690.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Development of a Gene Inactivation System for Bacteroides forsythus: Construction and Characterization of a BspA Mutant

Kiyonobu Honma,1 Howard K. Kuramitsu,1,2 Robert J. Genco,1,2 and Ashu Sharma1,*

Department of Oral Biology, School of Dental Medicine,1 and Department of Microbiology, School of Medicine and Biomedical Science,2 State University of New York at Buffalo, Buffalo, New York 14214

Received 6 October 2000/Returned for modification 14 December 2000/Accepted 17 April 2001

Bacteroides forsythus is a gram-negative anaerobic bacterium associated with periodontitis. The bspA gene encoding a cell surface associated leucine-rich repeat protein (BspA) involved in adhesion to fibronectin and fibrinogen was recently cloned from this bacterium in our laboratory. We now describe the construction of a BspA-defective mutant of B. forsythus. This is the first report describing the generation of a specific gene knockout mutant of B. forsythus, and this procedure should be useful in establishing the identity of virulence-associated factors in these organisms.

* Corresponding author. Mailing address: Department of Oral Biology, 211 Foster Hall, 3435 Main St., State University of New York at Buffalo, Buffalo, NY 14214. Phone: (716) 829-2759. Fax: (716) 829-3942. E-mail: sharmaa{at}buffalo.edu.

Infection and Immunity, July 2001, p. 4686-4690, Vol. 69, No. 7
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.7.4686-4690.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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