This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Melby, P. C.
Right arrow Articles by Cheng, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Melby, P. C.
Right arrow Articles by Cheng, J.

 Previous Article  |  Next Article 

Infection and Immunity, August 2001, p. 4719-4725, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.4719-4725.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Leishmania donovani p36(LACK) DNA Vaccine Is Highly Immunogenic but Not Protective against Experimental Visceral Leishmaniasis

Peter C. Melby,1,2,3,* Jue Yang,1 Weiguo Zhao,1 Luis E. Perez,1 and Jun Cheng1,dagger

Medical Service, Department of Veterans Affairs Medical Center, South Texas Veterans Health Care System,1 and Departments of Medicine2 and Microbiology,3 University of Texas Health Science Center, San Antonio, Texas

Received 10 January 2001/Returned for modification 22 February 2001/Accepted 1 May 2001

The acquisition of immunity following subclinical or resolved infection with the intracellular parasite Leishmania donovani suggests that vaccination could prevent visceral leishmaniasis (VL). The LACK (Leishmania homolog of receptors for activated C kinase) antigen is of interest as a vaccine candidate for the leishmaniases because of its immunopathogenic role in murine L. major infection. Immunization of mice with a truncated (24-kDa) version of the 36-kDa LACK antigen, delivered in either protein or DNA form, was found previously to protect against cutaneous L. major infection by redirecting the early T-cell response away from a pathogenic interleukin-4 (IL-4) response and toward a protective Th1 response. The amino acid sequence of the Leishmania p36(LACK) antigen is highly conserved, but the efficacy of this vaccine antigen in preventing disease caused by strains other than L. major has not been determined. We investigated the efficacy of a p36(LACK) DNA vaccine against VL because of the serious nature of this form of leishmaniasis and because it was unclear whether the LACK vaccine would be effective in a model where there was not a dominant pathogenic IL-4 response. We demonstrate here that although the LACK DNA vaccine induced a robust parasite-specific Th1 immune response (IFN-gamma but not IL-4 production) and primed for an in vivo T-cell response to inoculated parasites, it did not induce protection against cutaneous or systemic L. donovani challenge. Coadministration of IL-12 DNA with the vaccine did not enhance the strong vaccine-induced Th1 response or augment a protective effect.

* Corresponding author. Mailing address: Department of Medicine, Division of Infectious Diseases, University of Texas Health Science Center, 7703 Floyd Curl Dr., Mailcode 7881, San Antonio, TX 78229-3900. Phone: (210) 567-4614. Fax: (210) 567-4670. E-mail: melby{at}uthscsa.edu.

dagger Present address: Gene Therapy Research Center, Institute of infectious Diseases, The 302 Hospital of PLA, Beijing, China.

Infection and Immunity, August 2001, p. 4719-4725, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.4719-4725.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Samant, M., Gupta, R., Kumari, S., Misra, P., Khare, P., Kushawaha, P. K., Sahasrabuddhe, A. A., Dube, A. (2009). Immunization with the DNA-Encoding N-Terminal Domain of Proteophosphoglycan of Leishmania donovani Generates Th1-Type Immunoprotective Response against Experimental Visceral Leishmaniasis. J. Immunol. 183: 470-479 [Abstract] [Full Text]  
  • Bhowmick, S., Ravindran, R., Ali, N. (2008). gp63 in Stable Cationic Liposomes Confers Sustained Vaccine Immunity to Susceptible BALB/c Mice Infected with Leishmania donovani. Infect. Immun. 76: 1003-1015 [Abstract] [Full Text]  
  • Coler, R. N., Goto, Y., Bogatzki, L., Raman, V., Reed, S. G. (2007). Leish-111f, a Recombinant Polyprotein Vaccine That Protects against Visceral Leishmaniasis by Elicitation of CD4+ T Cells. Infect. Immun. 75: 4648-4654 [Abstract] [Full Text]  
  • Salay, G., Dorta, M. L., Santos, N. M., Mortara, R. A., Brodskyn, C., Oliveira, C. I., Barbieri, C. L., Rodrigues, M. M. (2007). Testing of Four Leishmania Vaccine Candidates in a Mouse Model of Infection with Leishmania (Viannia) braziliensis, the Main Causative Agent of Cutaneous Leishmaniasis in the New World. CVI 14: 1173-1181 [Abstract] [Full Text]  
  • Stober, C. B., Lange, U. G., Roberts, M. T. M., Alcami, A., Blackwell, J. M. (2007). Heterologous Priming-Boosting with DNA and Modified Vaccinia Virus Ankara Expressing Tryparedoxin Peroxidase Promotes Long-Term Memory against Leishmania major in Susceptible BALB/c Mice. Infect. Immun. 75: 852-860 [Abstract] [Full Text]  
  • Martins, D. R. A., Jeronimo, S. M. B., Donelson, J. E., Wilson, M. E. (2006). Leishmania chagasi T-Cell Antigens Identified through a Double Library Screen. Infect. Immun. 74: 6940-6948 [Abstract] [Full Text]  
  • Dondji, B., Perez-Jimenez, E., Goldsmith-Pestana, K., Esteban, M., McMahon-Pratt, D. (2005). Heterologous Prime-Boost Vaccination with the LACK Antigen Protects against Murine Visceral Leishmaniasis. Infect. Immun. 73: 5286-5289 [Abstract] [Full Text]  
  • Basu, R., Bhaumik, S., Basu, J. M., Naskar, K., De, T., Roy, S. (2005). Kinetoplastid Membrane Protein-11 DNA Vaccination Induces Complete Protection against Both Pentavalent Antimonial-Sensitive and -Resistant Strains of Leishmania donovani That Correlates with Inducible Nitric Oxide Synthase Activity and IL-4 Generation: Evidence for Mixed Th1- and Th2-Like Responses in Visceral Leishmaniasis. J. Immunol. 174: 7160-7171 [Abstract] [Full Text]  
  • Aguilar-Be, I., da Silva Zardo, R., Paraguai de Souza, E., Borja-Cabrera, G. P., Rosado-Vallado, M., Mut-Martin, M., del Rosario Garcia-Miss, M., Palatnik de Sousa, C. B., Dumonteil, E. (2005). Cross-Protective Efficacy of a Prophylactic Leishmania donovani DNA Vaccine against Visceral and Cutaneous Murine Leishmaniasis. Infect. Immun. 73: 812-819 [Abstract] [Full Text]  
  • Pinto, E. F., Pinheiro, R. O., Rayol, A., Larraga, V., Rossi-Bergmann, B. (2004). Intranasal Vaccination against Cutaneous Leishmaniasis with a Particulated Leishmanial Antigen or DNA Encoding LACK. Infect. Immun. 72: 4521-4527 [Abstract] [Full Text]  
  • Campbell, K., Diao, H., Ji, J., Soong, L. (2003). DNA Immunization with the Gene Encoding P4 Nuclease of Leishmania amazonensis Protects Mice against Cutaneous Leishmaniasis. Infect. Immun. 71: 6270-6278 [Abstract] [Full Text]  
  • Iborra, S., Soto, M., Carrion, J., Nieto, A., Fernandez, E., Alonso, C., Requena, J. M. (2003). The Leishmania infantum Acidic Ribosomal Protein P0 Administered as a DNA Vaccine Confers Protective Immunity to Leishmania major Infection in BALB/c Mice. Infect. Immun. 71: 6562-6572 [Abstract] [Full Text]  
  • Coelho, E. A. F., Tavares, C. A. P., Carvalho, F. A. A., Chaves, K. F., Teixeira, K. N., Rodrigues, R. C., Charest, H., Matlashewski, G., Gazzinelli, R. T., Fernandes, A. P. (2003). Immune Responses Induced by the Leishmania (Leishmania) donovani A2 Antigen, but Not by the LACK Antigen, Are Protective against Experimental Leishmania (Leishmania) amazonensis Infection. Infect. Immun. 71: 3988-3994 [Abstract] [Full Text]  
  • Davies, C. R, Kaye, P., Croft, S. L, Sundar, S. (2003). Leishmaniasis: new approaches to disease control. BMJ 326: 377-382 [Full Text]  
  • Ahmed, S., Colmenares, M., Soong, L., Goldsmith-Pestana, K., Munstermann, L., Molina, R., McMahon-Pratt, D. (2003). Intradermal Infection Model for Pathogenesis and Vaccine Studies of Murine Visceral Leishmaniasis. Infect. Immun. 71: 401-410 [Abstract] [Full Text]  
  • Afrin, F., Rajesh, R., Anam, K., Gopinath, M., Pal, S., Ali, N. (2002). Characterization of Leishmania donovani Antigens Encapsulated in Liposomes That Induce Protective Immunity in BALB/c Mice. Infect. Immun. 70: 6697-6706 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»